Transient receptor potential vanilloid 4 (TRPV4) expression on the nerve fibers of human dental pulp is upregulated under inflammatory condition
Transient receptor potential vanilloid 4 (TRPV4) expression on the nerve fibers of human dental pulp is upregulated under inflammatory condition
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DOI：
10.1016/j.archoralbio.2018.02.011
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发表时间：
2018-05-01
影响因子：
3
通讯作者：
Hossain, Mohammad Zakir
中科院分区：
文献类型：
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作者：
Bakri, Marina M.;Yahya, Farhana;Hossain, Mohammad Zakir
目的：瞬时受体电位香草酸 4 (TRPV4) 被认为是一种机械、温度和渗透压受体。在牙髓炎症条件下，牙齿在暴露于各种无害刺激时会变得敏感。本研究的目的是研究与炎症状况相关的无症状和有症状牙齿的人牙髓神经纤维上TRPV4通道的表达。设计：对拔除的人恒牙牙髓进行荧光免疫组织化学处理。本研究使用了 10 颗无症状（正常）和 10 颗有症状（与牙髓炎相关的症状）牙齿。通过标记蛋白基因产物 9.5 的免疫染色来鉴定神经纤维，并用 4',6-二脒基-2-苯基吲哚对细胞进行复染。使用抗TRPV4抗体来追踪TRPV4表达。结果：TRPV4表达与神经纤维标记物共定位。有症状牙齿神经中 TRPV4 的免疫反应性比正常牙齿更强烈 (p < 0.05)。与无症状（正常）牙齿相比，有症状牙齿牙髓中的共定位点数量显着增加（p < 0.05）。结论：人牙髓神经纤维上存在TRPV4通道的表达。我们的研究结果表明，在牙髓炎症条件下，TRPV4 表达上调。 TRPV4 通道的上调可能与炎症条件下牙髓对无害的机械、热和渗透刺激的过度反应有关。
Objective: Transient receptor potential vanilloid 4 (TRPV4) has been considered as a mechano-, thermo- and osmo-receptor. Under inflammatory conditions in dental pulp, teeth can become sensitive upon exposure to a variety of innocuous stimuli. The objective of the present study was to investigate the expression of the TRPV4 channel on nerve fibers in human dental pulp of non-symptomatic and symptomatic teeth associated with inflammatory conditions.Design: Dental pulp from extracted human permanent teeth was processed for fluorescence immunohistochemistry. Ten asymptomatic (normal) and 10 symptomatic (symptoms associated with pulpitis) teeth were used in this study. Nerve fibers were identified by immunostaining for a marker, protein gene product 9.5, and the cells were counterstained with 4',6-diamidino-2-phenylindole. An anti-TRPV4 antibody was used to trace TRPV4 expression.Results: TRPV4 expression was co-localized with the nerve fiber marker. Immunoreactivity for TRPV4 was more intense (p < 0.05) in the nerves of symptomatic teeth than those of normal teeth. The number of co-localization spots was increased significantly (p < 0.05) in the dental pulp of symptomatic teeth compared with that of asymptomatic (normal) teeth.Conclusions: There is expression of TRPV4 channels on the nerve fibers of human dental pulp. Our findings suggest upregulation of TRPV4 expression under inflammatory conditions in the pulp. The upregulation of TRPV4 channels may be associated with the exaggerated response of dental pulp to innocuous mechanical, thermal and osmotic stimuli under inflammatory conditions.