Development of diabetic nephropathy in nude mice
Development of diabetic nephropathy in nude mice
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DOI：
10.3275/8962
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发表时间：
2013-12-01
影响因子：
5.4
通讯作者：
Shang, W-Y.
中科院分区：
文献类型：
--
作者：
Lin, S.;Xu, P-C.;Shang, W-Y.
背景：免疫功能紊乱在糖尿病(DM)中十分常见。然而，这种免疫功能紊乱是否会影响糖尿病的发生发展，尤其是糖尿病肾病的发生发展，目前尚无证据。目的：探讨T细胞缺失对糖尿病肾病的影响。材料和方法：Balb/c裸鼠和Balb/c野生型裸鼠(WT)注射链脲佐菌素(STZ)。检测血清肿瘤坏死因子-α(TNF-α)、血糖、体重、尿白蛋白/肌酐比值、肾重/体重(KW/BW)。结果：造模后，裸鼠与WT小鼠血糖水平除2周时差异无统计学意义(28.3+/-4.9 mm o l/L vs 23.1+/-3.9 m o l/L，p<；0.01)。在第4周，裸鼠血清肿瘤坏死因子-α水平达到175.08+/-46.03pg/ml(P<；0.05，与基线水平80.19+/-8.46pg/ml相比)，而WT小鼠血清肿瘤坏死因子-α水平稳定。第4周裸鼠体重低于WT组(14.7±3.15gvs17.97+/-2.85g，p<；0.05)，尿白蛋白/肌酐比值(Alb/Cr值)高于WT组(50.96+/-5.57 mg/mmovs41.09+/-5.79 mg/mmos，p<；0.05)；裸鼠肾重/体重(0.01352+/-0.00163)高于WT小鼠(0.01173+/-0.00131，P<0.05)。相关分析显示，4周时尿Alb/Cr与血清肿瘤坏死因子-α水平呈正相关(r=0.588，p<；0.01)。在第4周，糖尿病裸小鼠肾小球中IV型胶原的增加比糖尿病WT小鼠更明显(p<；0.05)。结论：糖尿病患者T细胞缺失可能影响糖尿病肾病的发生发展。(J.内分泌。投资。36：938-943,2013)(C)Editrice Kurtis
Background: Immune dysfunction is very common in diabetes mellitus (DM). However, there is no evidence whether such immune dysfunction can influence the development of DM, especially the development of diabetic nephropathy (DN). Aim: To investigate the influence of absence of T cells on DN. Materials and Methods: Balb/c nude mice and Balb/c wild-type nude (WT) mice were injected with streptozotocin (STZ). Serum tumor necrosis factor a (TNF-alpha), blood glucose, body weight, urine albumin/creatinine ratio and rate of kidney weight to body weight (KW/BW) were measured. Results: After modeling, there was no difference of blood glucose level between nude mice and WT mice except at week 2 (28.3 +/- 4.9 mmol/l vs 23.1 +/- 3.9 mmol/l, p < 0.01). At week 4, the serum TNF-alpha level of nude mice got to 175.08 +/- 46.03 pg/ml (p < 0.05, compared with baseline level 80.19 +/- 8.46 pg/ml), whereas the TNF-alpha levels of WT mice was stable. At week 4, the body weight of nude mice was lower than that of WT mice (14.7 +/- 3.15 g vs 17.97 +/- 2.85 g, p < 0.05); the urine albumin/creatinine ratio (Alb/Cr) of nude mice was higher than that of WT mice (50.96 +/- 5.57 mg/mmol vs 41.09 +/- 5.79 mg/mmol, p < 0.05); the kidney weight to body weight of nude mice was higher than that of WT mice (0.01352 +/- 0.00163 vs 0.01173 +/- 0.00131, p < 0.05). Correlation analysis showed urine Alb/Cr positively correlated with serum TNF-a level at week 4 (r=0.588, p < 0.01). At week 4, the increase of type IV collagen in the glomeruli was more prominent in diabetic nude mice than in diabetic WT mice (p < 0.05). Conclusions: Absence of T cells in DM might influence the development of DN. (J. Endocrinol. Invest. 36: 938-943, 2013) (C) Editrice Kurtis