Selective immunosuppression resulting from exposure to the carcinogenic congener of benzopyrene in B6C3F1 mice.
Selective immunosuppression resulting from exposure to the carcinogenic congener of benzopyrene in B6C3F1 mice.
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B6C3F1 小鼠暴露于苯并芘致癌同源物导致选择性免疫抑制。
DOI：
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发表时间：
1983
影响因子：
4.6
通讯作者：
L. Lawson
中科院分区：
文献类型：
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作者：
J. Dean;M. Luster;G. Boorman;L. Lauer;R. W. Leubke;L. Lawson
将B6 C3 F1小鼠暴露于苯并芘的两种同系物，致癌物苯并（a）芘（B（a）P）或非致癌物苯并（e）芘（B（e）P）。小鼠暴露于B（a）P导致T依赖性（TD）抗原SRBC的IgM和IgG抗体空斑形成细胞（PFC）数量减少，以及T非依赖性（TI）抗原LPS的IgM PFC数量减少。使用TNP-LPS检测对半抗原缀合的TI抗原的IgM应答，用于较不成熟的B细胞（B1）的反应性，使用TNP-Ficoll检测对较成熟的B细胞（B2）的反应性。暴露于B（a）P严重抑制TNP-Ficoll PFC反应高达77%，而不改变TNP-LPS反应。这些数据表明，暴露于B（a）P改变了成熟B细胞对TD和B2 TI抗原的分化和抗体产生。暴露于B（e）P后，未观察到PFC的变化。Mishell-Dutton共培养证实B细胞受到影响，而T辅助细胞或抑制性Mphi细胞不受影响。B（a）P处理小鼠的细胞介导的免疫活性参数，包括对KLH的迟发性皮肤超敏反应、同种异体移植物或肿瘤细胞排斥以及对单核细胞增生李斯特菌的敏感性均未改变。
B6C3F1 mice were exposed to two congeners of benzopyrene, either the carcinogen benzo(a)pyrene (B(a)P) or the non-carcinogen benzo(e)pyrene (B(e)P. Exposure of mice to B(a)P resulted in a reduced number of IgM and IgG antibody plaque forming cells (PFC) to the T-dependent (TD) antigen SRBC and IgM PFC's to the T-independent (TI) antigen LPS. The IgM response to hapten conjugated TI antigens was examined using TNP-LPS for reactivity of less mature B cells (B1) and TNP-Ficoll for more mature B cells (B2). Exposure to B(a)P severely depressed the TNP-Ficoll PFC response by up to 77% without altering the TNP-LPS response. These data indicated that exposure to B(a)P alters differentiation and antibody production in mature B cells to both TD and B2 TI antigens. No change in PFC was observed following exposure to B(e)P. Mishell-Dutton co-cultures confirmed that B cells were affected and that T helper cells or suppressor Mphi were not involved. Parameters of cell-mediated immunocompetence including delayed cutaneous hypersensitivity to KLH, allograft or tumour cell rejection and susceptibility to Listeria monocytogens were unaltered in B(a)P treated mice.