Safflower polysaccharides activate the transcription factor NF-κB via Toll-like receptor 4 and induce cytokine production by macrophages
Safflower polysaccharides activate the transcription factor NF-κB via Toll-like receptor 4 and induce cytokine production by macrophages
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DOI：
10.1016/s1567-5769(02)00076-0
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发表时间：
2002-07-01
影响因子：
5.6
通讯作者：
Nagai, K
中科院分区：
文献类型：
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作者：
Ando, I;Tsukumo, Y;Nagai, K
从红花（Carthamus tinctorius L.）刺激腹腔巨噬细胞合成多种细胞因子。在许多细胞类型中，SF 1和SF 2诱导转录因子NF-κ B激活所必需的I κ B α的快速降解。Toll样受体4（TLR 4），而不是TLR 2，表达在所有细胞系，响应SF 1和SF 2。TLR 4和MD-2的增强表达使对SF 1和SF 2的反应性增强。此外，这些红花多糖不能诱导从Tlr 4基因中具有点突变的C3 H/HeJ小鼠制备的腹腔巨噬细胞产生TNF-α和NO。因此，这些观察清楚地表明红花多糖通过TLR 4激活NF-κ B信号通路。(C)2002 Elsevier Science B. V.保留所有权利。
Two active polysaccharide fractions (SF1 and SF2) purified from dried safflower petals (Carthamus tinctorius L.) stimulated the synthesis of various cytokines by peritoneal macrophages. In a number of cell types, SF1 and SF2 induced a rapid degradation Of IkappaBalpha essential for the activation of the transcription factor NF-kappaB. Toll-like receptor 4 (TLR4), but not TLR2, was expressed in all cell lines that responded to SF1 and SF2. Enforced expression of TLR4 and MD-2 rendered responsiveness to SF1 and SF2. Moreover, these safflower polysaccharides failed to induce the production of TNF-alpha and NO by peritoneal macrophages prepared from C3H/HeJ mice that have a point mutation in the Tlr4 gene. Thus, these observations clearly indicate that safflower polysaccharides activate the NF-kappaB signaling pathway via TLR4. (C) 2002 Elsevier Science B.V. All rights reserved.