The prevalence of sequences homologous to IS256 in clinical enterococcal isolates.
The prevalence of sequences homologous to IS256 in clinical enterococcal isolates.
复制标题
临床肠球菌分离株中与 IS256 同源的序列的普遍性。
作者：
Louis B. Rice;Anna S. Thorisdottir
使用斑点杂交技术和内部IS 256探针，我们筛选了103个临床肠球菌分离株的IS 256同源序列的存在。大多数筛选的分离株表现出耐一种或多种抗菌药物。总体而言，在88/103（85%）分离株中证实与内部IS 256探针杂交。49/53株（92%）庆大霉素耐药菌株与IS 256探针杂交。此外，34/45（76%）庆大霉素敏感的aph 2”（-）菌株具有与IS 256同源的序列。Southern杂交实验表明，IS 256基因在庆大霉素敏感菌株中经常以多拷贝存在。这些结果表明，IS 256是高度流行的临床肠球菌分离株，我们可以预期出现新的，IS 256为基础的复合移动的元素。
Using dot blot hybridization techniques and an internal IS256 probe, we screened 103 clinical enterococcal isolates for the presence of sequences homologous to IS256. Most screened isolates exhibited resistance to one or more antimicrobial agents. Overall, hybridization to the internal IS256 probe was demonstrable in 88/103 (85%) isolates. 49/53 (92%) gentamicin-resistant isolates hybridized with the IS256 probe. In addition, 34/45 (76%) gentamicin-susceptible, aph2"(-) strains possessed sequences homologous to IS256. Southern hybridization experiments indicated that IS256 was frequently present in multiple copies in gentamicin-susceptible strains. These results suggest that IS256 is highly prevalent in clinical enterococcal isolates and that we may anticipate the emergence of novel, IS256-based composite mobile elements.