A DREPP protein interacted with PeaT1 from Alternaria tenuissima and is involved in elicitor-induced disease resistance in Nicotiana plants
A DREPP protein interacted with PeaT1 from Alternaria tenuissima and is involved in elicitor-induced disease resistance in Nicotiana plants
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DREPP 蛋白与 Alternaria tenuissima 的 PeaT1 相互作用，并参与烟草植物中诱导子诱导的抗病性
DOI：
10.1007/s10265-018-1038-5
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发表时间：
2018-05
影响因子：
2.8
通讯作者：
Qiu Dewen
中科院分区：
文献类型：
--
作者：
Meng Fanlu;Xiao Yao;Guo Lihua;Zeng Hongmei;Yang Xiufen;Qiu Dewen
PeaT1是一种蛋白质激发子，来自真菌病原体细穗孢霉。我们的前期研究表明，该诱导子可以诱导包括烟草在内的多种植物的防御反应，增强其抗病能力。然而，PeaT1引发防御反应的免疫激活机制尚不清楚。在本研究中，使用FITC（异硫氰酸荧光素）标记法评估了激发子蛋白PeaT1与质膜之间的关联。通过125i -PeaT1交联和Far Western blot分析分离到一个PeaT1相互作用蛋白，命名为PtBP1 （PeaT1 Binding protein 1）。通过质谱分析和生物信息学分析，推测22 kDa的质膜蛋白PtBP1是DREPP （developmental regulated plasma membrane polypeptide，发育调节质膜多肽）家族的成员，该家族在逆境条件下被诱导，可能参与下游信号传导。为了进一步验证这种关联，我们进行了Far Western blot、共免疫沉淀和双分子荧光互补（BiFC）分析，结果表明PtBP1在体内和体外都能与PeaT1结合。病毒诱导的基因沉默（VIGS）分析显示，与对照烟草响尾蛇病毒（TRV）相比，PeaT1处理后，PtBP1沉默降低了烟草花叶病毒（TMV）抗性。
PeaT1 is a proteinaceous elicitor from fungal pathogen Alternaria tenuissima. Our previous research revealed that this elicitor could induce defense response and enhance disease resistance in various plants including Nicotiana plants. However, immune activation mechanisms whereby PeaT1 elicits defense response remain unclear. In this study, the association between elicitor protein PeaT1 and the plasma membrane was assessed using the FITC (Fluorescein isothiocyanate) labeling method. A PeaT1-interacting protein was isolated via125I-PeaT1 cross-linking and Far Western blot analyses, and designated PtBP1 (PeaT1 Binding Protein 1). From the data of Mass spectrometry (MS) and bioinformatics analysis, the 22 kDa plasma membrane protein PtBP1 was inferred to be a member of DREPP (developmentally regulated plasma membrane polypeptide) family that is induced in plants under stress conditions and might get involved in downstream signaling. For further verification of this association, Far Western blot, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) analyses were performed, showing PtBP1 could bind with PeaT1 in vitro and in vivo. Virus-induced gene silencing (VIGS) analysis exhibited that PtBP1 silencing in Nicotiana benthamiana attenuated tobacco mosaic virus (TMV) resistance compared to the tobacco rattle virus (TRV) control after PeaT1 treatment.
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影响因子：
5.1
作者：
Li JF;Park E;von Arnim AG;Nebenführ A
通讯作者：
Nebenführ A
影响因子：
7.2
作者：
Waadt, Rainer;Schmidt, Lena K.;Kudla, Joerg
通讯作者：
Kudla, Joerg
MDP25 是一种新型钙调节蛋白，通过破坏拟南芥皮质微管的稳定性来介导下胚轴细胞伸长
影响因子：
11.6
作者：
Li, Jiejie;Wang, Xianling;Mao, Tonglin
通讯作者：
Mao, Tonglin
影响因子：
4.9
作者：
Kato, Mariko;Nagasaki-Takeuchi, Nahoko;Maeshima, Masayoshi
通讯作者：
Maeshima, Masayoshi
影响因子：
14.9
作者：
ASLANIDIS, C;DEJONG, PJ
通讯作者：
DEJONG, PJ