Fabrication of Microwell Arrays Based on Two-Dimensional Ordered Polystyrene Microspheres for High-Throughput Single-Cell Analysis
Fabrication of Microwell Arrays Based on Two-Dimensional Ordered Polystyrene Microspheres for High-Throughput Single-Cell Analysis
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DOI：
10.1021/ac102094r
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发表时间：
2010-11-15
影响因子：
7.4
通讯作者：
Lin, Jin-Ming
中科院分区：
文献类型：
--
作者：
Liu, Chuansen;Liu, Jiangjiang;Lin, Jin-Ming
本文描述了一种在聚二甲基硅氧烷（PDMS）中通过模塑单层有序聚苯乙烯（PS）微球制备圆底微孔阵列（MA）的方法。PS微球在载玻片上自组装，并在240 ℃下主要从底部部分熔融，以增加与基底的粘合力。部分熔融的PS阵列用作母版以产生MA。微孔尺寸在10-20 μ m范围内可调，具有圆形底部;这样的3D结构不容易通过传统的软光刻获得。粘附和非粘附细胞类型都可以高效地保留在微孔中。为了证明用该MA进行实时细胞筛选的优点，还对捕获的单细胞进行了单细胞酶动力学分析。PDMS MA可以在高通量药物筛选、细胞簇的引导形成和多细胞通信中找到应用。
This paper describes a method of fabricating rounded bottom microwell arrays (MA) in poly(dimethylsiloxane) (PDMS) by molding a monolayer of ordered polystyrene (PS) microspheres. PS microspheres were self-assembled on a glass slide and partially melted mainly from the bottom at 240 degrees C to increase adhesive force with the substrate. The partially melted PS arrays were used as master to generate MA. Microwell sizes are tunable in the 10-20 mu m range with rounded bottoms; such a 3D structure is not readily obtainable through conventional soft lithography. Both adherent and nonadherent cell types can be retained in the microwells with high efficiency. As a demonstration of the advantage of real-time cell screening with this MA, single cell enzyme kinetic analysis was also carried out on trapped single cells. The PDMS MA may find applications in high-throughput drug screening, guided formation of cell clusters, and multicellular communication.