Upregulated expression of transforming growth factor-beta, type IV collagen, and plasminogen activator inhibitor-1 mRNA are decreased after release of unilateral ureteral obstruction.
Upregulated expression of transforming growth factor-beta, type IV collagen, and plasminogen activator inhibitor-1 mRNA are decreased after release of unilateral ureteral obstruction.
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DOI：
10.1620/tjem.197.159
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发表时间：
2002-07
期刊：
影响因子：
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通讯作者：
Y. Ogata;S. Ishidoya;A. Fukuzaki;H. Kaneto;A. Takeda;C. Ohyama;S. Orikasa;Y. Arai
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文献类型：
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作者：
Y. Ogata;S. Ishidoya;A. Fukuzaki;H. Kaneto;A. Takeda;C. Ohyama;S. Orikasa;Y. Arai
肾小管间质纤维化是梗阻性肾损害的主要原因。解除梗阻对梗阻肾的影响尚不清楚。我们研究输尿管梗阻解除对肾纤维化及纤维化因子表达的影响。大鼠单侧输尿管梗阻（UUO） 5天。通过去除封闭橡胶管释放梗阻后，形态学评估间质体积的变化，并通过逆转录聚合酶链反应（RT-PCR）检测转化生长因子- β (tgf - β)、IV型胶原（collagen IV）和纤溶酶原激活物抑制剂-1 (PAI-1) mRNA的表达，持续28天。梗阻解除后7 ~ 28天，肾间质体积、IV型胶原蛋白和PAI-1 mRNA逐渐减少。然而，tgf - β mRNA的表达增加持续了14天，然后在释放后28天下降。总之，梗阻诱导的肾纤维化随着tgf - β和胶原IV的表达降低而恢复。梗阻后肾脏中PAI-1表达的降低可能有助于细胞外基质蛋白的降解和输尿管梗阻解除后小管间质纤维化的恢复，至少部分如此。
Tubulointerstitial fibrosis is a major cause of irreversible renal damage in the obstructed kidney. The effects of release of obstruction on the obstructed kidney are not clearly understood. We investigated the effects of the release of ureteral obstruction on renal fibrosis and the expression of fibrogenic factors. Rats underwent 5 day of unilateral ureteral obstruction (UUO). After release of obstruction by removing an encased rubber tube, changes in interstitial volume were morphologically evaluated and the mRNA expression of transforming growth factor-beta (TGF-beta), type IV collagen (collagen IV), and plasminogen activator inhibitor-1 (PAI-1) were examined by reverse transcription-polymerase chain reaction (RT-PCR) up to 28 days. Renal interstitial volume, collagen IV and PAI-1 mRNA gradually decreased from 7 days to 28 days after release of obstruction. However, increased expression of TGF-beta mRNA persisted up to 14 days, and then declined 28 days after release. In conclusion, obstruction-induced renal fibrosis was recovered with diminished expression of TGF-beta and collagen IV. Decreased PAI-1 expression in the post-obstructed kidney may contribute to the degradation of extracellular matrix proteins and recovery of tubulointerstitial fibrosis, at least partly, after release of ureteral obstruction.