Phylogenetic diversity of bacteria associated with the marine sponge Rhopaloeides odorabile
Phylogenetic diversity of bacteria associated with the marine sponge Rhopaloeides odorabile
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DOI：
10.1128/aem.67.1.434-444.2001
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发表时间：
2001-01-01
影响因子：
4.4
通讯作者：
Hill, RT
中科院分区：
文献类型：
--
作者：
Webster, NS;Wilson, KJ;Hill, RT
采用分子技术记录了与海绵体散发气味有关的微生物多样性。对克隆的DNA片段进行16S rRNA测序，评估海绵相关菌的系统发育亲缘关系。荧光原位杂交（FISH）证实了16S rDNA分析显示的优势基团的存在。群落结构极其多样，有放线菌门、低g +C革兰氏阳性菌、变形菌门的β -亚区和γ -亚区、Cytophaga/Flavobacterium、绿硫菌、绿非硫菌、植物菌等序列类型的代表，没有已知的近亲。FISH探针揭示了这些细菌在海绵组织中的空间位置，在某些情况下提示可能的共生功能。来自新型放线菌的高比例16S rRNA序列很好地证明了r.o odorabile中存在本土海洋放线菌组合。该文库有70个代表，克隆重复率低，微生物多样性高。通过16S rRNA分析确定海绵相关微生物的系统发育关系，有助于合理选择培养基和分离条件，针对具有代表性的特定细菌群进行实验室培养。采用含有海绵提取物的新型培养基分离以前未从海绵中回收的细菌。
Molecular techniques were employed to document the microbial diversity associated with the marine sponge Rhopaloeides odorabile. The phylogenetic affiliation of sponge-associated bacteria was assessed by 16S rRNA sequencing of cloned DNA fragments. Fluorescence in situ hybridization (FISH) was used to confirm the presence of the predominant groups indicated by 16S rDNA analysis. The community structure was extremely diverse with representatives of the Actinobacteria, low-G+C gram-positive bacteria, the beta- and gamma -subdivisions of the Proteobacteria, Cytophaga/Flavobacterium, green sulfur bacteria, green nonsulfur bacteria, planctomycetes, and other sequence types with no known close relatives. FISH probes revealed the spatial location of these bacteria within the sponge tissue, in some cases suggesting possible symbiotic functions. The high proportion of 16S rRNA sequences derived from novel actinomycetes is good evidence for the presence of an indigenous marine actinomycete assemblage in R. odorabile. High microbial diversity was inferred from low duplication of clones in a library with 70 representatives. Determining the phylogenetic affiliation of sponge-associated microorganisms by 16S rRNA analysis facilitated the rational selection of culture media and isolation conditions to target specific groups of well-represented bacteria for laboratory culture. Novel media incorporating sponge extracts were used to isolate bacteria not previously recovered from this sponge.