Persistence of a xylE marker gene in Pseudomonas putida introduced into soils of differing texture
Persistence of a xylE marker gene in Pseudomonas putida introduced into soils of differing texture
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引入不同质地土壤的恶臭假单胞菌中 xylE 标记基因的持久性
DOI：
10.1099/00221287-138-4-667
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
A. O'donnell
中科院分区：
文献类型：
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作者：
S. Macnaughton;D. A. Rose;A. O'donnell
摘要：对两种基因工程微生物 (GEM) 恶臭假单胞菌菌株 PaW8 的生长和存活进行了研究，该菌株含有标记质粒 pLV1013 (IncQ) 或 pLV1016 (IncP)，两者都含有 xylE 基因，在从砂壤土到泥炭的土壤中研究了两种基因工程微生物 (GEM) 的生长和存活情况。对 89 天内可行的 GEM 进行了计数。衰变动力学分析表明，通过 xylE 基因的持久性监测，GEM 的存活率在沙壤土和粘土壤土中呈双相。这两种生物在快速死亡阶段之前都显示出在泥炭土中初始生长的证据。结果表明，土壤质地对转基因恶臭假单胞菌的存活有影响，随着粘土含量的增加，死亡减少。
Summary: The growth and survival of two genetically engineered micro-organisms (GEMs), Pseudomonas putida strain PaW8 containing either marker plasmid pLV1013 (IncQ) or pLV1016 (IncP), both of which contain the xylE gene, were investigated in soils ranging in texture from a sandy loam to a peat. Viable GEMs were enumerated over a period of 89 d. Analysis of the decay kinetics indicated that GEM survival, as monitored by the persistence of the xylE gene, was biphasic in the sandy and clay loams. Both organisms showed evidence of initial growth in the peat soil prior to a rapid death phase. The results indicated that soil texture has an effect on the survival of genetically engineered P. putida, death decreasing with increasing clay content.