Mobility of two optional G + C-rich clusters of the var1 gene of yeast mitochondrial DNA.
Mobility of two optional G + C-rich clusters of the var1 gene of yeast mitochondrial DNA.
复制标题
酵母线粒体 DNA var1 基因的两个可选富含 G C 簇的迁移率。
DOI：
10.1093/genetics/126.1.53
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Perlman,PS
中科院分区：
文献类型：
--
作者：
Wenzlau,JM;Perlman,PS
酵母mtDNA包含两种不同的可移动可选序列，两个I族内含子和一个短的富含G + c的var1基因插入。杂交中各元素的移动被称为基因转换，但G + C簇转换的机制尚不清楚。在capensis Saccharomyces mtDNA中发现了一个新的var1基因等位基因，可以更详细地比较内含子迁移和G + C簇转换。酿酒葡萄球菌var1基因缺乏所有酿酒葡萄球菌var1基因中存在的cc+元素和前面描述的可选a+元素。在与cc+ a-和cc+ a+酿酒酵母菌株的杂交中，发现这两个簇都是可移动的，而在后一种杂交中，似乎是独立转化的，只转化到同源插入位点。没有证据表明侧翼标记共转换（内含子转换的一个标志性特征），尽管在集群转换位点的两侧都有附近的物理标记。这些数据表明G + C簇转换与内含子迁移只有表面上的相似之处；类比原核生物转位机制被考虑。
Yeast mtDNA contains two different kinds of mobile optional sequences, two group I introns and a short G + C-rich insertion to some var1 genes. Movement of each element in crosses has been called gene conversion though little is known about the mechanism of G + C cluster conversion. A new allele of the var1 gene found in mtDNA of Saccharomyces capensis is described that permitted a more detailed comparison between intron mobility and G + C cluster conversion. The S. capensis var1 gene lacks the cc+ element present in all S. cerevisiae var 1 genes and the previously described optional a+ element. In crosses with cc+ a- and cc+ a+ S. cerevisiae strains, both clusters were found to be mobile and, in the latter cross, appear to convert independently and only to homologous insertion sites. No evidence for flanking marker coconversion (a hallmark feature of intron conversion) was obtained despite the availability of nearby physical markers on both sides of cluster conversion sites. These data indicate that G + C cluster conversion has only a superficial resemblance to intron mobility; analogies to procaryotic transposition mechanisms are considered.