Developmental and tissue-specific expression of JIP-23, a jasmonate-inducible protein of barley.
Developmental and tissue-specific expression of JIP-23, a jasmonate-inducible protein of barley.
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JIP-23（一种大麦茉莉酸诱导蛋白）的发育和组织特异性表达。
DOI：
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发表时间：
1996
影响因子：
4.9
通讯作者：
C. Wasternack
中科院分区：
文献类型：
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作者：
B. Hause;U. Demus;Christiane Teichmann;B. Parthier;C. Wasternack
通过测量发芽过程中转录物和蛋白质的时间依赖性积累，研究了大麦叶（Hordeum vulgare cv. Salome）23 kDa 茉莉酸诱导蛋白（JIP-23）的发育表达。分别通过免疫细胞化学和原位杂交分析 JIP-23 的组织特异性表达。在种子萌发期间，发现 JIP-23 mRNA 短暂积累，并在 32 小时达到最大值，而蛋白质在表达开始后可稳定检测到。与茉莉酸处理的叶子相比，发芽期间 JIP-23 新亚型的出现表明，大麦的 JIP-23 基因家族能够根据发育阶段表达不同亚型的亚型。 JIP-23及其转录物主要存在于6天龄幼苗的盾片、盾片结节和初生叶的下部。所有这些组织都表现出高水平的内源性茉莉酸。原位杂交揭示了盾片结节板韧皮部伴细胞中 JIP-23 mRNA 的特异性积累。据此，在根的韧皮部细胞以及盾片结节和盾片的薄壁细胞中通过免疫细胞化学方法检测到JIP-23。 JIP-23 的细胞类型特异性发生仅限于已知因活性溶质转运而受到高渗透压的细胞。这一观察结果表明，这种蛋白质的表达可能是对发育过程中渗透压的反应。
Developmental expression of a 23 kDa jasmonate-induced protein (JIP-23) of barley leaves (Hordeum vulgare cv. Salome) was studied by measuring the time-dependent accumulation of transcript and protein during germination. Tissue-specific expression of JIP-23 was analyzed immunocytochemically and by in situ hybridizations, respectively. During seed germination JIP-23 mRNA was found to accumulate transiently with a maximum at 32 h, whereas the protein was steadily detectable after the onset of expression. The occurrence of new isoforms of JIP-23 during germination in comparison to jasmonate-treated leaves suggests, that the JIP-23 gene family of barley is able to express different subsets of isoforms dependent on the developmental stage. JIP-23 and its transcript were found mainly in the scutellum, the scutellar nodule and in lower parts of the primary leaf of 6 days old seedings. All these tissues exhibited high levels of endogenous jasmonates. In situ hybridization revealed specific accumulation of JIP-23 mRNA in companion cells of the phloem in the nodule plate of the scutellum. In accordance with that, JIP-23 was detected immunocytochemically in phloem cells of the root as well as of the scutellar nodule and in parenchymatic cells of the scutellum. The cell type-specific occurrence of JIP-23 was restricted to cells, which are known to be highly stressed osmotically by active solute transport. This observation suggests, that the expression of this protein might be a response to osmotic stress during development.