Polymorphisms in CYP-7A1, not APOE, influence the change in plasma lipids in response to population dietary change in an 8 year follow-up;: results from the Czech MONICA study
Polymorphisms in CYP-7A1, not APOE, influence the change in plasma lipids in response to population dietary change in an 8 year follow-up;: results from the Czech MONICA study
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DOI：
10.1016/s0009-9120(03)00025-0
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发表时间：
2003-06-01
影响因子：
2.8
通讯作者：
Talmud, PJ
中科院分区：
文献类型：
--
作者：
Hubacek, JA;Pitha, J;Talmud, PJ
目的：探讨载脂蛋白E （APOE; epsilon2、epsilon3、epsilon4）和胆固醇-7 - α羟化酶（CYP-7A1; -204A—>C）基因变异对血脂水平变化的影响。设计与方法：131名膳食成分发生显著变化的男性，总胆固醇从1988年的6.21 +/- 1.31 mmol/L下降到1996年的5.43 +/- 1.06 mmol/L。经过8年的随访研究。用PCR方法分析多态性。结果：APOE基因型影响血浆总胆固醇和LDL胆固醇，其中epsilon4携带者水平最高，epsilon2携带者水平最低，1988年达到临界意义（p = 0.06）， 1996年强烈影响LDL胆固醇水平（p = 0.008）。然而，APOE并没有随着时间的推移影响这些指标的变化。相比之下，CYP-7A1 - 204a ->C多态性本身并不影响脂质测量，但与血浆总胆固醇的下降密切相关[AA -0.38 (+/- 0.20) mmol/L, AC -0.65 +/- (0.08), CC -1.33 (+/- 0.3) mmol/L, p = 0.01]。结论：cyp7a1基因的变异可能在个体对饮食成分的敏感性中起重要作用。(C) 2003加拿大临床化学学会。版权所有。
Objectives: To evaluate the influence of variation in the genes for apolipoprotein E (APOE; epsilon2, epsilon3, epsilon4) and cholesterol-7alpha hydroxylase (CYP-7A1; -204A-->C) on plasma lipid level changes.Design and methods: 131 males for whom dietary composition markedly changed and total cholesterol decreased (from 6.21 +/- 1.31 mmol/L in 1988 - 5.43 +/- 1.06 mmol/L in 1996). over an 8 yr follow-up study. Polymorphisms were investigated using PCR.Results: APOE genotype influenced plasma total and LDL cholesterol, with carriers of the epsilon4 having the highest and epsilon2 carriers the lowest levels, this reached borderline significance for cholesterol in 1988 (p = 0.06) and strongly affected the 1996 levels of LDL cholesterol (p = 0.008). However, APOE did not influence the change in these measures over time. In contrast, the CYP-7A1 -204A-->C polymorphism did not affect lipid measures per se but was strongly associated with a dec tease in plasma total cholesterol [AA -0.38 (+/- 0.20) mmol/L, AC -0.65 +/- (0.08), CC -1.33 (+/- 0.3) mmol/L, p = 0.01] over the 8 yr time period.Conclusions: Variation in the CYP-7A1 gene may play an important role in an individual's sensitivity to dietary composition. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.