SEQUENCE ORGANIZATION AND GENOMIC DISTRIBUTION OF THE MAJOR FAMILY OF INTERSPERSED REPEATS OF MOUSE DNA
SEQUENCE ORGANIZATION AND GENOMIC DISTRIBUTION OF THE MAJOR FAMILY OF INTERSPERSED REPEATS OF MOUSE DNA
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DOI：
10.1073/pnas.79.2.355
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
BERNARDI, G
中科院分区：
文献类型：
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作者：
MEUNIERROTIVAL, M;SORIANO, P;BERNARDI, G
研究了小鼠基因组中一个分散的DNA重复序列家族的组织和分布。这些重复序列的长度至少为5600个碱基对（bp），包含两个连续的BamHI内切酶片段，大小分别为4000和540 bp，其中较大的片段包括先前作者研究过的一个1350 bp的EcoRI片段。这些重复序列在它们的限制图谱中是多态的，代表了小鼠基因组中散布重复序列的主要家族。这些重复序列几乎只存在于小鼠DNA的2个较轻的主要成分中，它们的大BamHI片段的碱基组成与这些成分相匹配。重复序列的基因组分布与结构基因不同，它们不仅存在于小鼠DNA的2个轻组分中，也存在于小鼠DNA的2个重组分中。重复序列不参与基因表达的调节，至少不以任何简单的方式参与。
The organization and distribution of a family of interspersed DNA repeats in the mouse genome was investigated. The repeats are at least 5600 base pairs (bp) in size and contain 2 contiguous BamHI endonuclease fragments, 4000 and 540 bp in size, the larger of which includes a 1350-bp EcoRI fragment studied by previous authors. The repeats are polymorphic in their restriction maps, and represent the major family of interspersed repeats in the mouse genome. The repeats are present almost exclusively in the 2 light major components of mouse DNA, and the base composition of their large BamHI fragments matches that of those components. The genomic distribution of the repeats is different from that of structural genes, which are present not only in the 2 light components but also in the 2 heavy components of mouse DNA. The repeats are not involved, at least in any simple way, in the regulation of gene expression.