Anti-Inflammatory Effect of Auraptene Extracted from Trifoliate Orange (Poncirus Trifoliate) on LPS-Stimulated RAW 264.7 Cells
Anti-Inflammatory Effect of Auraptene Extracted from Trifoliate Orange (Poncirus Trifoliate) on LPS-Stimulated RAW 264.7 Cells
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DOI：
10.1007/s10753-013-9695-y
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发表时间：
2013-12-01
期刊：
影响因子：
5.1
通讯作者：
Deng, Xiuxin
中科院分区：
文献类型：
--
作者：
Yan, Huiqing;Ma, Zhaocheng;Deng, Xiuxin
三叶Poncirus trifoliate是一种长期用于治疗炎症相关疾病的传统药用植物，而三叶Poncirus trifoliate orange含有丰富的auraptene。本研究通过与阿司匹林作为阳性对照组比较，评价auraptene作为一种潜在的抗炎药，并探讨auraptene对脂多糖（LPS）刺激的RAW 264.7细胞上的前列腺素E-2 （PGE(2)）和环氧合酶2 （COX-2）的作用机制。采用酶联免疫吸附试验、逆转录聚合酶链反应、实时荧光定量PCR、western-blotting等方法。结果表明，auraptene具有较好的生物相容性和较低的细胞毒性。同时显著抑制lps刺激的巨噬细胞产生PGE(2)。实验组COX-2 mRNA表达量较高，COX-2蛋白表达量相对较低，说明auraptene抑制了COX-2蛋白的转录后表达，但没有抑制转录过程。与阿司匹林相比，auraptene较低的细胞毒性使其成为一种潜在的药物来源，可以使患有慢性炎症性疾病并需要长期药物治疗的患者受益。
Poncirus trifoliate is a traditional Chinese medicinal plant used for treating inflammation-related diseases for a long time and trifoliate orange contains abundant auraptene. The present study was to evaluate auraptene as a potential anti-inflammatory agent and investigate the mechanism of auraptene against prostaglandins E-2 (PGE(2)) and cyclooxygenase-2 (COX-2) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells by comparing it with aspirin as a positive control group. The methods of enzyme-linked immunosorbent assay, reverse transcriptive polymerase chain reaction, real-time PCR, and western-blotting were used in the study. The results showed that auraptene exhibited better biocompatibility and lower cytotoxicity. At the same time, it significantly inhibited the production of PGE(2) on LPS-stimulated macrophage cells. The auraptene-treated group had a higher COX-2 mRNA expression but relatively lower COX-2 protein level which implied that auraptene suppressed the post-transcriptional expression of COX-2 protein but not the transcriptional process. Compared with aspirin, the lower cytotoxicity of auraptene can make it a potential source for medicine that can benefit patients who are suffering from chronic inflammatory diseases and need long-term medication.