Genome-wide identification and expression analysis of the NAC transcription factor family in tomato (Solanum lycopersicum) during aluminum stress
Genome-wide identification and expression analysis of the NAC transcription factor family in tomato (Solanum lycopersicum) during aluminum stress
复制标题
铝胁迫下番茄 NAC 转录因子家族的全基因组鉴定和表达分析
DOI：
10.1186/s12864-020-6689-7
复制
发表时间：
2020-04-07
期刊：
影响因子：
4.4
通讯作者：
Yang, Jian Li
中科院分区：
文献类型：
--
作者：
Jin, Jian Feng;Wang, Zhan Qi;Yang, Jian Li
NAC蛋白家族（NAM、ATAF 1/2和CUC 2）是一类植物特异性转录因子。然而，尽管番茄基因组已被破译多年，但番茄NAC基因的鉴定和功能研究仍未见报道。本研究的目的是确定NAC基因家族，并探讨其潜在的作用，在应对铝stress.Results93 NAC基因被确定，并根据其染色体位置命名。系统发育分析发现，SNAC广泛分布于5个类群。基因表达分析表明，SlNAC在果实不同组织和不同发育阶段有不同的表达水平。放线菌酮处理和qRT-PCR分析表明，S1 NACs可能有助于调节番茄对铝胁迫的响应，其中19个在铝胁迫后显著上调或下调。结论本研究为进一步研究S1 NACs在番茄中的生物学功能奠定了基础，并将有助于今后番茄农艺性状的改良。
BackgroundThe family of NAC proteins (NAM, ATAF1/2, and CUC2) represent a class of large plant-specific transcription factors. However, identification and functional surveys ofNACgenes of tomato (Solanum lycopersicum) remain unstudied, despite the tomato genome being decoded for several years. This study aims to identify theNACgene family and investigate their potential roles in responding to Al stress.ResultsNinety-threeNACgenes were identified and named in accordance with their chromosome location. Phylogenetic analysis foundSlNACsare broadly distributed in 5 groups. Gene expression analysis showed thatSlNACshad different expression levels in various tissues and at different fruit development stages. Cycloheximide treatment and qRT-PCR analysis indicated thatSlNACsmay aid regulation of tomato in response to Al stress, 19 of which were significantly up- or down-regulated in roots of tomato following Al stress.ConclusionThis work establishes a knowledge base for further studies on biological functions ofSlNACsin tomato and will aid in improving agricultural traits of tomato in the future.