Molecular Cloning of cDNA Encoding Two Types of Pituitary Gonadotropin α Subunit from the Goldfish,Carassius auratus
Molecular Cloning of cDNA Encoding Two Types of Pituitary Gonadotropin α Subunit from the Goldfish,Carassius auratus
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金鱼垂体促性腺激素α亚基两种cDNA的分子克隆
DOI：
10.1006/gcen.1996.6840
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发表时间：
1997
影响因子：
2.7
通讯作者：
K. Aida
中科院分区：
文献类型：
--
作者：
Makito Kobayashi;Y. Kato;Y. Yoshiura;K. Aida
摘要利用聚合酶链反应（PCR）技术从金鱼垂体cDNA文库中克隆了两种编码垂体促性腺激素（GTH）α亚基的cDNA。金鱼GTH-α亚基cDNA分别命名为α1和α2，编码117和118个氨基酸，包括一个23个氨基酸的信号肽。这两种cDNA在核苷酸和氨基酸序列上具有很高的同源性，但在α1中存在一个三联体核苷酸的缺失。推导的氨基酸序列代表α1和α2亚基之间的4个残基的差异。对金鱼基因组DNA的分析表明，每只金鱼都有两个不同的GTH-α1和-α2亚基相关基因。RT-PCR分析结果表明，这两个GTH-α亚基基因在不同的性别和性腺成熟度下表达水平不同。
Abstract Two types of cDNA encoding the α subunit of pituitary gonadotropin (GTH) were cloned by polymerase chain reaction (PCR) for goldfish pituitary cDNA library. The goldfish GTH-α subunit cDNAs, designated as α1 and α2, encode 117 and 118 amino acids, respectively, including a 23-amino-acid signal peptide. These two types of cDNAs showed high homology in nucleotide and amino acid sequence, but deletion of a triplet nucleotides was present in α1. Deduced amino acid sequences represented differences in four residues between α1 and α2 subunits. Analyses of goldfish genomic DNA revealed that each individual goldfish possesses two distinct genes relevant to GTH-α1 and -α2 subunits. Results of reverse transcriptase-PCR analysis suggest that these two GTH-α subunit genes are expressed at different levels without relation to sex and gonadal maturity.