Self-quenching of uranin: Instrument response function for color sensitive photo-detectors
Self-quenching of uranin: Instrument response function for color sensitive photo-detectors
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DOI：
10.1016/j.jlumin.2010.07.027
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发表时间：
2010-12-01
影响因子：
3.6
通讯作者：
Gryczynski, Ignacy
中科院分区：
文献类型：
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作者：
Luchowski, Rafal;Sabnis, Sushant;Gryczynski, Ignacy
浓度是有机荧光团荧光特性的关键决定因素。我们研究了聚乙烯醇（PVA）薄膜中二钠荧光素（铀）荧光的自猝灭。亮度和各向异性的浓度依赖性变化伴随着寿命的缩短。我们发现浓度为 0.54 M 时，寿命降低至 7 Ps。在浓度为 0.18 M 时，寿命为 10 ps，量子产率相对较高，为 0.002。在这些条件下，荧光强度衰减是均匀的（很好地近似于单个寿命）。我们意识到这样的样品是光谱学和显微镜的理想荧光寿命标准，因此表征了时域技术的仪器响应函数。我们证明，自猝灭铀素可以实现不受颜色效应影响的测量，使其成为比散射光更好的终身参考选择。 (C) 2010 Elsevier B.V. 保留所有权利。
Concentration is a key determining factor in the fluorescence properties of organic fluorophores. We studied self-quenching of disodium fluorescein (uranin) fluorescence in polyvinyl alcohol (PVA) thin films. The concentration dependent changes in brightness and anisotropy were followed by a lifetime decrease. We found that at a concentration of 0.54 M, the lifetime decreases to 7 Ps. At a concentration of 0.18 M the lifetime was 10 ps with the relatively high quantum yield of 0.002. In these conditions the fluorescence intensity decay was homogeneous (well approximated by a single lifetime). We realized that such a sample was an ideal fluorescence lifetime standard for spectroscopy and microscopy, and therefore characterized instrument response functions for a time-domain technique. We show that self-quenched uranin enables measurements free of the color effect, making it a superior choice for a lifetime reference over scattered light. (C) 2010 Elsevier B.V. All rights reserved.