Structure of the (+)-CC-1065-DNA adduct: critical role of ordered water molecules and implications for involvement of phosphate catalysis in the covalent reaction.
Structure of the (+)-CC-1065-DNA adduct: critical role of ordered water molecules and implications for involvement of phosphate catalysis in the covalent reaction.
复制标题
( )-CC-1065-DNA 加合物的结构：有序水分子的关键作用以及对磷酸盐催化参与共价反应的影响。
DOI：
10.1021/bi00229a002
复制
发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Hurley,LH
中科院分区：
文献类型：
--
作者：
Lin,CH;Beale,JM;Hurley,LH
材料和方法化学品。（+）-CC-1065得自Upjohn Company，不经进一步纯化即使用。[l70]水（60和45原子% 170）购自剑桥同位素实验室。用于制备NMR缓冲液的试剂磷酸钠（99.99%）和氯化钠（99.99%）购自Aldrich。HPLC用水和甲醇分别购自巴克斯特科技公司和Fisher公司。用于纯化12-mer双链体和12-mer加合物的羟基磷灰石购自Calbiochem。Sephadex G-25（超细）购自Pharmacia。
Materials and MethodsChemicals.(+)-CC-1065 was obtained from The Upjohn Company and used without further purification.[l70] Water (60 and 45 atom% 170) was purchased from Cambridge Isotope Laboratories. Reagents used to prepare the NMR buffer, sodium phosphate (99.99%), and sodium chloride (99.99%) were purchased from Aldrich. HPLC water and methanol were purchased from Baxter Scientific and Fisher, respectively. Hydroxylapatite used to purify the 12-mer duplex and the 12-mer adduct was purchased from Calbiochem. Sephadex G-25 (superfine) was purchased from Pharmacia.