Echinococcus multilocularis Infection, Southern Ontario, Canada
Echinococcus multilocularis Infection, Southern Ontario, Canada
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DOI：
10.3201/eid2502.180299
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发表时间：
2019-02-01
影响因子：
11.8
通讯作者：
Peregrine, Andrew S.
中科院分区：
文献类型：
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作者：
Kotwa, Jonathon D.;Isaksson, Mats;Peregrine, Andrew S.
泡状棘球蚴病是由多房棘球绦虫中间阶段感染引起的疾病，如果不治疗，通常会导致人和狗的死亡。自2012年以来，在加拿大安大略南部的5只狗、3只狐猴和1只花栗鼠中诊断出泡状棘球蚴病，该地区以前被认为没有这些绦虫。由于人类和动物的健康问题，我们估计了整个安大略南部野生犬科动物的感染率。在2015-2017年期间，我们在尸检期间收集了460只野生犬科动物（416只郊狼，44只狐狸）的粪便样本，并使用半自动磁性捕获探针DNA提取和实时PCR方法对它们进行了分析。多室DNA令人惊讶的是，23%（95% CI 20%-27%）的样本检测呈阳性。通过使用空间扫描测试，我们确定了一个感染集群（相对风险2.26; p = 0.002）在中西部地区的省。该集群包括人口密集地区，表明人畜共患病传播。
Alveolar echinococcosis, the disease caused by infection with the intermediate stage of the Echinococcus multilocularis tapeworm, is typically fatal in humans and dogs when left untreated. Since 2012, alveolar echinococcosis has been diagnosed in 5 dogs, 3 lemurs, and 1 chipmunk in southern Ontario, Canada, a region previously considered free of these tapeworms. Because of human and animal health concerns, we estimated prevalence of infection in wild canids across southern Ontario. During 2015-2017, we collected fecal samples from 460 wild canids (416 coyotes, 44 foxes) during postmortem examination and analyzed them by using a semiautomated magnetic capture probe DNA extraction and real-time PCR method for E. multilocularis DNA. Surprisingly, 23% (95% CI 20%-27%) of samples tested positive. By using a spatial scan test, we identified an infection cluster (relative risk 2.26; p = 0.002) in the western-central region of the province. The cluster encompasses areas of dense human population, suggesting zoonotic transmission.