Photo-driven highly efficient one-step CO2 biomethanation with engineered photo-synthetic bacteria rhodopseudomonas palustris
Photo-driven highly efficient one-step CO2 biomethanation with engineered photo-synthetic bacteria rhodopseudomonas palustris
复制标题
利用工程光合细菌沼泽红假单胞菌进行光驱动高效一步二氧化碳生物甲烷化
DOI：
10.1021/acssuschemeng.0c02703
复制
发表时间：
2020
影响因子：
8.4
通讯作者：
Yang-Chun Yong
中科院分区：
文献类型：
--
作者：
Liqin Ma;Zhen Fang;Yan-Zhai Wang;Jun Zhou;Yang-Chun Yong
CO2methanation is considered as a promising process for methane production and renewable energy storage. However, current methane production processes involve nonrenewable energy input or high temperature and pressure, which raises the concerns of sustainability. Here, a sustainable photo-driven one-step CO2biomethanation approach using whole-cell biocatalysis was established by endowing the methanation capability to a photosynthetic bacterium (PSB). With simple genetic engineering of inherent dinitrogen reductase, the CO2biomethanation PSB was constructed from facultative anaerobic bacteria ofRhodopseudomonas palustrisCGMCC 1.2180. With this engineered PSB strain, one-step CO2biomethanation powered by sustainable solar energy was realized at ambient temperature and pressure. After optimization, a record-high photo-driven bio-CH4yield (CO2-to-CH4, 35% with 10 μmol/L CO2) was achieved. This study provided an efficient and sustainable methane-producing approach, demonstrating the great potential of bacterial methanation for CO2transformation and solar energy harvesting.
