Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia
Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia
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DOI：
10.1016/j.parint.2021.102404
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发表时间：
2021-06-23
影响因子：
1.9
通讯作者：
Munkhjargal, Tserendorj
中科院分区：
文献类型：
--
作者：
Bilegjargal, Janchivsengee;Rzad, Izabella;Munkhjargal, Tserendorj
骆驼丝虫病是一种重要的寄生虫病，对世界各地的骆驼产业造成经济影响。然而，蒙古双峰驼的丝虫病研究尚未见报道。因此，本研究的目的是在蒙古国南部和西南部三个省的双峰驼中检测和鉴定伊凡斯疟原虫微丝虫。采集了400只不同年龄和性别的健康双峰骆驼的血液样本。使用各种诊断技术对所有血液样本进行分析。采用Knott技术在30头双峰驼（7.5%）中检出微丝蚴，其中13头双峰驼（3.3%）经直接涂片检测呈阳性。PCR法在18头双峰驼（4.5%）中检出伊文氏杆菌。5岁以下年龄组的双峰驼患病率较高，而5-10岁年龄组和10岁以上年龄组的双峰驼阳性率最低。为了确认形态鉴定，对伊文氏疟原虫coi基因序列进行了系统发育分析。蒙古双峰骆驼和伊朗单峰骆驼的伊文氏疟原虫coi基因序列完全一致，在系统发育上聚类在一起。本文首次报道了蒙古国双峰驼中伊文氏弓形虫微丝虫的分子检测和鉴定。
Cameline filarosis is an important parasitic disease having an economic impact on the camel industry around the world. However, there has been no study on filarosis in Bactrian camels of Mongolia. Therefore, the aim of the present study was to detect and identify microfilariae of Deraiophoronema evansi (D. evansi) in Bactrian camels from three provinces, located in southern and southwestern Mongolia. Blood samples were obtained from 400 healthy two-humped camels of different ages and both sexes. All blood samples were analysed using a variety of diagnostic techniques. Microfilariae were detected in 30 Bactrian camels (7.5%) by the Knott technique, while 13 Bactrian camels (3.3%) tested positive in a direct smear test. D. evansi was detected in 18 Bactrian camels (4.5%) by PCR assay. Prevalence was shown to be high among Bactrian camels in the age group up to 5 years, while the lowest positive results were obtained for Bactrian camels in the 5-10-year age group and the over 10-year age group. To confirm the morphological identification, D. evansi-COI gene sequences were subjected to phylogenetic analyses. The D. evansi-COI gene sequences from Mongolian two-humped camels were identical to sequences from Iranian one-humped camels and were clustered together with these sequences in the phylogeny. This is the first report of molecular detection and identification of microfilariae of D. evansi in Bactrian camels of Mongolia.