A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.
A novel two-component, expandable bioadhesive for exposed defect coverage: Applicability to prenatal procedures.
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DOI：
10.1016/j.jpedsurg.2020.09.030
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发表时间：
2021-01
影响因子：
2.4
通讯作者：
Fauza DO
中科院分区：
文献类型：
--
作者：
Lazow SP;Labuz DF;Freedman BR;Rock A;Zurakowski D;Mooney DJ;Fauza DO
我们试图测试一种新型可膨胀生物粘附复合材料的选定特性，该复合材料可以增强液体环境中的粘附控制。兔胎 (n=23) 在妊娠第 22-25 天（足月 32-33 天）接受了脊柱裂手术。缺陷立即用双组分坚韧粘合剂覆盖，该粘合剂由水凝胶组成，该水凝胶由离子交联藻酸盐和共价交联聚丙烯酰胺的双网络制成，与桥接壳聚糖聚合物粘合剂连接。动物在足月前被安乐死以进行不同的分析，包括液压测试。随着程序的优化，水凝胶在 70% (16/23) 的恢复胎儿以及所有最后 14 个胎儿中保持粘附。安乐死时，粘附水凝胶的面积中位数增加了两倍（IQR：1.7-2.4），超声和组织学证实了缺陷覆盖。修复失败的中位最大压力为 15mmHg (IQR:7.8–55.3)，超过报道的新生儿脑脊液压力。这种新型生物粘附复合材料可以将藻酸盐-聚丙烯酰胺水凝胶选择性、稳定地附着到胎兔模型中脊柱裂缺陷的特定区域，而水凝胶会随着缺陷的推移而膨胀。它可能成为脊柱裂和其他先天性异常的产前修复的有价值的替代方案。
We sought to test select properties of a novel, expandable bioadhesive composite that allows for enhanced adhesion control in liquid environments. Rabbit fetuses (n=23) underwent surgical creation of spina bifida on gestational day 22–25 (term 32–33 days). Defects were immediately covered with a two-component tough adhesive consisting of a hydrogel made of a double network of ionically crosslinked alginate and covalently crosslinked polyacrylamide linked to a bridging chitosan polymer adhesive. Animals were euthanized prior to term for different analyses, including hydraulic pressure testing. Hydrogels remained adherent in 70% (16/23) of the recovered fetuses and in all of the last 14 fetuses as the procedure was optimized. Adherent hydrogels showed a median two-fold (IQR:1.7–2.4) increase in area at euthanasia, with defect coverage confirmed by ultrasound and histology. The median maximum pressure to repair failure was 15mmHg (IQR:7.8–55.3), exceeding reported neonatal cerebrospinal fluid pressures. This novel bioadhesive composite allows for selective, stable attachment of an alginate-polyacrylamide hydrogel to specific areas of the spina bifida defect in a fetal rabbit model, while the hydrogel expands with the defect over time. It could become a valuable alternative for the prenatal repair of spina bifida and possibly other congenital anomalies.
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影响因子：
13.6
作者：
Blacklow, S. O.;Li, J.;Mooney, D. J.
通讯作者：
Mooney, D. J.
影响因子：
2.8
作者：
Wilks, Alex M.;Rabice, Sarah R.;Smith, Andrew M.
通讯作者：
Smith, Andrew M.
DOI：
10.1126/science.aah6362
发表时间：
2017-07-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li J;Celiz AD;Yang J;Yang Q;Wamala I;Whyte W;Seo BR;Vasilyev NV;Vlassak JJ;Suo Z;Mooney DJ
通讯作者：
Mooney DJ
影响因子：
3.4
作者：
Zhao X
通讯作者：
Zhao X
影响因子：
1.4
作者：
Danzer, E.;Finkel, R. S.;Johnson, M. P.
通讯作者：
Johnson, M. P.