Adaptive optical fluorescence microscopy

Adaptive optical fluorescence microscopy
复制标题

DOI:
10.1038/nmeth.4218
复制
发表时间:
2017-04-01
期刊:
影响因子:
48
通讯作者:
Ji, Na
Ji, Na
中科院分区:
生物学1区
文献类型:
--
作者:
Ji, Na

文献摘要

被引文献

相似文献

在过去的四分之一世纪见证了快速发展的荧光显微镜技术,使结构和功能成像的生物标本在前所未有的深度和分辨率。然而,这些方法在多细胞生物体中的性能被样品诱导的光学像差所降低。在这里,我回顾了最近关于整合自适应光学的工作,自适应光学是一种最初应用于天文望远镜以对抗大气像差的技术,以提高生物成像荧光显微镜的图像质量。
The past quarter century has witnessed rapid developments of fluorescence microscopy techniques that enable structural and functional imaging of biological specimens at unprecedented depth and resolution. The performance of these methods in multicellular organisms, however, is degraded by sample-induced optical aberrations. Here I I I review recent work on incorporating adaptive optics, a technology originally applied in astronomical telescopes to combat atmospheric aberrations, to improve image quality of fluorescence microscopy for biological imaging.