Microwave-assisted hydrothermal preparation using adenosine 5′-triphosphate disodium salt as a phosphate source and characterization of zinc-doped amorphous calcium phosphate mesoporous microspheres
Microwave-assisted hydrothermal preparation using adenosine 5′-triphosphate disodium salt as a phosphate source and characterization of zinc-doped amorphous calcium phosphate mesoporous microspheres
复制标题
DOI：
10.1016/j.micromeso.2013.06.020
复制
发表时间：
2013-11
影响因子：
5.2
通讯作者：
J. Zhao;Ying-Jie Zhu;Jian-Qiang Zheng;Feng Chen;Jin Wu
中科院分区：
文献类型：
--
作者：
J. Zhao;Ying-Jie Zhu;Jian-Qiang Zheng;Feng Chen;Jin Wu
以CaCl2、zncl2和5′-三磷酸腺苷二钠盐（ATP）为生物相容性有机磷源，采用微波辅助水热法制备了锌掺杂无定形磷酸钙（Zn/ACP）介孔微球。报道了ATP和Zn2+作为水溶液中无定形磷酸钙（ACP）的稳定剂。在本研究中，我们发现ATP是在水溶液中稳定ACP的主要因素，并在ACP中掺杂Zn2+离子以达到抗菌效果。采用x射线粉末衍射（XRD）、扫描电镜（SEM）、氮气吸附-解吸等温线和孔径分布、傅里叶变换红外光谱（FTIR）和热重（TG）分析对制备的Zn/ACP介孔微球进行了表征。热重分析（TG）和红外光谱（FTIR）结果表明，锌/ACP介孔微球表面吸附有残余的ATP分子。锌/ACP介孔微球对细菌金黄色葡萄球菌和大肠杆菌的ph敏感性和良好的抑菌活性表明，所制备的锌/ACP介孔微球在生物医学领域具有广阔的抗菌应用前景。
Zinc-doped amorphous calcium phosphate (Zn/ACP) mesoporous microspheres have been prepared using CaCl2, ZnCl2and adenosine 5′-triphosphate disodium salt (ATP) as a biocompatible organic phosphorus source by a microwave-assisted hydrothermal method. Both ATP and Zn2+are reported as the stabilizer for amorphous calcium phosphate (ACP) in aqueous solution. In this study, we have found that ATP is the main factor for stabilizing ACP in aqueous solution and Zn2+ions are doped in ACP for the antibacterial benefit. X-ray powder diffraction (XRD), scanning electron microscopy (SEM), nitrogen adsorption–desorption isotherm and pore size distribution, Fourier transform infrared (FTIR) spectroscopy and thermogravimetry (TG) analysis are used to characterize the as-prepared Zn/ACP mesoporous microspheres. The TG and FTIR results reveal that some residual ATP molecules are adsorbed on the surface of the Zn/ACP mesoporous microspheres. The pH-sensitive Zn2+ion release behavior and good antibacterial activity of Zn/ACP mesosporous microspheres against bacteriaStaphylococcus aureusandEscherichia coliindicate that the as-prepared Zn/ACP mesoporous microspheres are promising for the antibacterial application in the biomedical fields.