‘Degraded’ RNA profiles in Arthropoda and beyond
‘Degraded’ RNA profiles in Arthropoda and beyond
复制标题
节肢动物及其他动物的“退化”RNA 谱
作者：
S. McCarthy;Michel M. Dugon;A. Power
对高质量/未降解 RNA 的要求对于一系列分子生物学分析至关重要。当分析来自藤壶 Lepas anatifera（节肢动物门，甲壳亚门）的 rRNA 完整性时，在生物分析仪电泳图上观察到明显降解的非典型或次优 rRNA 图谱。随后发现，rRNA 并未被降解，而是由于 28S rRNA 中的“缺口缺失”（也称为“隐藏断裂”）而产生。该位点的明显切除导致 28S rRNA 在热变性条件下断裂并与 18S rRNA 一起迁移，表面上呈现出“降解”的外观。对文献的审查显示，少数较早的昆虫研究也有类似的观察结果。然而，跨多个学科的阅读表明，这是一个在动物界内外都发生的更广泛的问题。目前的研究表明，28S rRNA 异常现象远远超出了节肢动物门的昆虫范围，并且在该门中广泛存在。我们确认这种异常与热转化有关，因为在热变性样品中观察到了间隙缺失模式，但在甲醛变性的凝胶中却没有观察到。
The requirement for high quality/non-degraded RNA is essential for an array of molecular biology analyses. When analysing the integrity of rRNA from the barnacle Lepas anatifera (Phylum Arthropoda, Subphylum Crustacea), atypical or sub-optimal rRNA profiles that were apparently degraded were observed on a bioanalyser electropherogram. It was subsequently discovered that the rRNA was not degraded, but arose due to a ‘gap deletion’ (also referred to as ‘hidden break’) in the 28S rRNA. An apparent excision at this site caused the 28S rRNA to fragment under heat-denaturing conditions and migrate along with the 18S rRNA, superficially presenting a ‘degraded’ appearance. Examination of the literature showed similar observations in a small number of older studies in insects; however, reading across multiple disciplines suggests that this is a wider issue that occurs across the Animalia and beyond. The current study shows that the 28S rRNA anomaly goes far beyond insects within the Arthropoda and is widespread within this phylum. We confirm that the anomaly is associated with thermal conversion because gap-deletion patterns were observed in heat-denatured samples but not in gels with formaldehyde-denaturing.
曼氏血吸虫 28S rRNA 基因中 54 核苷酸间隙区域的表征。
影响因子：
1.5
作者：
vanKeulen,H;Mertz,PM;LoVerde,PT;Shi,H;Rekosh,DM
通讯作者：
Rekosh,DM