Molecular analysis of tick-borne protozoan and rickettsial pathogens in small ruminants from two South African provinces
Molecular analysis of tick-borne protozoan and rickettsial pathogens in small ruminants from two South African provinces
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DOI：
10.1016/j.parint.2017.11.002
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发表时间：
2018-04-01
影响因子：
1.9
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
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作者：
Ringo, Aaron Edmond;Moumouni, Paul Franck Adjou;Xuan, Xuenan
壁虱传播的原生动物和立克次体疾病是非洲热带和亚热带地区牲畜的主要威胁。本研究调查了自由州和夸祖鲁-纳塔尔省绵羊和山羊中泰勒氏菌、绵羊巴贝斯虫、绵羊无浆体、吞噬细胞无浆菌、瘤胃埃立克次体和SFG立克次体的存在和分布。在这项研究中，总共筛选了91份血液样本，其中61份来自山羊，30份来自绵羊。利用泰勒氏菌(Theileria spp.)18S rRNA、绵羊巴贝斯虫(Bossu RRNA)、绵羊无浆体(AoMSP4)、无浆体吞噬细胞型1、瘤胃埃立克氏菌pCS20和SFG Rickettsia OmpA。泰勒氏菌、羊无浆体和瘤胃埃利希菌的总感染率分别为18例(19.8%)、33例(36.3%)和13例(14.3%)。两种病原体混合感染率为18.7%(17/91)，山羊的混合感染率高于绵羊。系统进化树分析表明，本研究的反胃埃希菌pCS20基因序列与Kumm2和Riverside南非株同源。绵羊泰勒虫SSU rRNA的系统树具有较长的分支长度，与其他序列相比，大部分来自亚洲和中东。这项研究为了解研究区硬虱传播疾病的发生情况提供了重要的数据，并有望改进这些疾病的诊断和控制方法。
Tick-borne protozoan and rickettsial diseases are a major threat to livestock in tropical and sub-tropical regions of Africa. In this study we investigated the presence and distribution of Theileria spp., Babesia ovis, Anaplasma ovis, Anaplasma phagocytophilum, Ehrlichia ruminantium and SFG Rickettsia in sheep and goats from Free State and KwaZulu-Natal provinces. A total of 91 blood samples were screened in this study, 61 from goats and 30 from sheep. PCR assay was conducted using primers based on Theileria spp. 18S rRNA, Babesia ovis (BoSSU rRNA), Anaplasma ovis (AoMSP4), Anaplasma phagocytophilum epank1, Ehrlichia ruminantium pCS20 and SFG Rickettsia OmpA. Overall infection rates of Theileria spp., Anaplasma ovis and Ehrlichia ruminantium were 18 (19.8%), 33 (36.3%) and 13 (14.3%), respectively. The co-infection of two pathogens were detected in 17/91 (18.7%) of all samples, goats having higher rates of co-infection compared to sheep. Phylogenetic tree analysis sequence of pCS20 gene of E. ruminantium of this study was found to be in the same clade with Kumm2 and Riverside strains both from South Africa. The phylogram of SSU rRNA of Theileria ovis had longer branch length compared to all other sequences most of which were from Asia and Middle East. This study provides important data for understanding the tick-borne diseases occurrence in the study area and it is expected to improve the approach for the diagnosis and control of these diseases.