The restorative effect of taurine on experimental nonalcoholic steatohepatitis
The restorative effect of taurine on experimental nonalcoholic steatohepatitis
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DOI：
10.1007/s10620-006-9359-y
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Xie, Wei-Fen
中科院分区：
文献类型：
--
作者：
Chen, Si-Wen;Chen, Yue-Xiang;Xie, Wei-Fen
我们的目的是探讨牛磺酸对实验性非酒精性脂肪性肝炎 (NASH) 的恢复作用。将36只SID大鼠随机分为3组，每组12只：正常组饲喂标准大鼠饲料；模型组和治疗组均给予高脂大鼠饲料喂养12周，治疗组同时皮下注射牛磺酸8周。观察肝脏组织学变化；通过免疫组织化学法鉴定TNF-α和TGF-β(1)蛋白表达； RT-PCR检测TNF-α、TGF-β(1)、I型前胶原和脂联素的mRNA表达；测量体重、体重增加、肝脏重量和肝脏指数；监测的生化参数包括血清转氨酶、血脂、空腹血糖和肝脏氧化应激水平。模型组大鼠肝脏重量、肝脏指数、血清转氨酶活性、血清甘油三酯、空腹血糖、氧化应激明显升高；与正常组相比，TNF-α、TGF-β(1)、I型前胶原的mRNA表达量明显增加，脂联素的表达量明显减少。模型组在组织学上观察到NASH典型的肝脏病变。牛磺酸治疗导致肝脏重量、肝脏指数、血清转氨酶活性、血清甘油三酯、空腹血糖和氧化应激显着降低；与模型组相比,TNF-α、TGF-β(1)、I型前胶原mRNA表达量下降,而脂联素表达量较模型组显着升高。在治疗组中观察到组织学改善。综上所述，牛磺酸可抑制脂质过氧化，改善脂质和糖代谢，减少TNF-α和TGF-β(1)的合成，促进脂联素的合成，对实验性NASH具有恢复作用。
Our objective was to explore the restorative effect of taurine on experimental nonalcoholic steatohepatitis (NASH). Thirty-six SID rats were randomly divided into three groups, 12 in each group: the normal group was fed standard rat diet; the model group and the treatment group were both fed a high-fat rat diet for 12 weeks, and the rats in the treatment group were simultaneously injected with taurine subcutaneously for 8 weeks. Hepatic histological change was observed; TNF-alpha and TGF-beta(1) protein expression was identified by immunohistochemistry; mRNA expression of TNF-alpha, TGF-beta(1), type I procollagen, and adiponectin was measured by RT-PCR; body weight, weight gain, liver weight, and liver index were measured; and biochemical parameters monitored included serum transaminases, serum lipids, fasting plasma glucose, and hepatic level of oxidative stress. Rats in the model group showed a significant increase in liver weight, liver index, serum transaminase activities, serum triglyceride, fasting plasma glucose, and oxidative stress; the mRNA expression of TNF-alpha, TGF-beta(1), and type I procollagen increased, whereas the expression of adiponectin decreased significantly, compared with that in the normal group. The typical hepatic lesions of NASH were observed histologically in the model group. Taurine treatment resulted in a significant decrease in liver weight, liver index, serum transaminase activities, serum triglyceride, fasting plasma glucose, and oxidative stress; the mRNA expression of TNF-alpha, TGF-beta(1), and type I procollagen decreased, but the expression of adiponectin increased significantly, compared with that in the model group. Histological improvement was observed in the treatment group. In conclusion, taurine could inhibit lipid peroxidation, improve lipid and glucose metabolism, decrease synthesis of TNF-alpha and TGF-beta(1), promote synthesis of adiponectin, and have a restorative effect on experimental NASH.