Extracellular matrix assembly and organization during zebrafish gastrulation
Extracellular matrix assembly and organization during zebrafish gastrulation
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DOI：
10.1016/j.matbio.2009.10.002
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发表时间：
2010-03-01
期刊：
影响因子：
6.9
通讯作者：
Jessen, Jason R.
中科院分区：
文献类型：
--
作者：
Latimer, Andrew;Jessen, Jason R.
斑马鱼原肠胚形成需要形态发生细胞运动，形成身体计划，并产生具有明确的前后轴和背腹轴的胚胎。调节这些细胞运动的是多种信号通路和蛋白质，包括wnt、src家族酪氨酸激酶、钙粘蛋白和基质金属蛋白酶。虽然我们对这些蛋白质如何影响细胞极性和迁移的知识在过去十年中有了很大的进展，但几乎没有关于斑马鱼原肠胚形成过程中细胞外基质（ECM）组织的数据。在这里，我们首次描述了早期斑马鱼胚胎中含有ECM的纤维连接蛋白（FN）和层粘连蛋白的组装。这种基质最早在原肠胚形成早期（表观代谢率65%）以小点的形式被发现，这些小点位于组织边界，将胚层和下层的卵黄细胞分开。纤维形成在原肠形成中期（80%）后增加，与平面细胞极性通路依赖的趋同和扩展细胞运动时期一致。我们发现存在于深层中胚层细胞下的FN原纤维在膜突出形成的方向排列。利用反义morpholino寡核苷酸，我们进一步发现FN表达的敲低会导致收敛和扩展缺陷。综上所述，我们的数据表明，与两栖动物胚胎相似，斑马鱼原肠胚中ECM的形成是一个动态过程，至少一部分极化细胞行为平行发生，形成胚胎体计划。这些结果为揭示ECM结构与调节收敛和扩展的细胞过程（如定向迁移和中外侧/径向嵌入）之间的相互关系提供了一个框架。(C) 2009 Elsevier B.V.版权所有
Zebrafish gastrulation entails morphogenetic cell movements that shape the body plan and give rise to an embryo with defined anterior-posterior and dorsal-ventral axes. Regulating these cell movements are diverse signaling pathways and proteins including Wnts, Src-family tyrosine kinases, cadherins, and matrix metalloproteinases. While our knowledge of how these proteins impact cell polarity and Migration has advanced considerably in the last decade, almost no data exist regarding the organization of extracellular matrix (ECM) during zebrafish gastrulation. Here, we describe for the first time the assembly of a fibronectin (FN) and laminin containing ECM in the early zebrafish embryo. This matrix was first detected at early gastrulation (65% epiboly) in the form of punctae that localize to tissue boundaries separating germ layers from each other and the underlying yolk cell. Fibrillogenesis increased after mid-gastrulation (80% epiboly) coinciding with the period of planar cell polarity pathway-dependent convergence and extension cell movements. We demonstrate that FN fibrils present beneath deep mesodermal cells are aligned in the direction of membrane protrusion formation. Utilizing antisense morpholino oligonucleotides, we further show that knockdown of FN expression causes a convergence and extension defect. Taken together, our data show that similar to amphibian embryos, the formation of ECM in the zebrafish gastrula is a dynamic process that occurs in parallel to at least a portion of the polarized cell behaviors shaping the embryonic body plan. These results provide a framework for uncovering the interrelationship between ECM structure and cellular processes regulating convergence and extension such as directed migration and mediolateral/radial intercalation. (C) 2009 Elsevier B.V. All rights reserved.