Spin1z induces the male pathway in the chicken by down-regulating Tcf4
Spin1z induces the male pathway in the chicken by down-regulating Tcf4
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Spin1z 通过下调 Tcf4 诱导鸡的雄性途径
DOI：
10.1016/j.gene.2021.145521
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发表时间：
2021-03-03
期刊：
影响因子：
3.5
通讯作者：
Li, Bichun
中科院分区：
文献类型：
--
作者：
Jiang, Jingyi;Zhang, Chen;Li, Bichun
SPINDLIN1-Z (SPIN1Z) 是 Spin/Ssty（Y 连锁精子发生特异性转录本）蛋白家族的成员，参与早期胚胎发育过程。我们之前的RNA-seq分析表明Spin1z在雄性胚胎干细胞（ESC）和原始生殖细胞（PGC）中大量表达，我们推测Spin1z可能在鸡雄性分化中发挥重要作用。因此，功能丧失和获得的实验提供了确凿的证据，证明 Spin1z 对于启动鸡的雄性发育是必要且充分的。此外，进行染色质免疫沉淀（ChIP）测定和双荧光素酶测定进一步证实Spin1z通过抑制Tcf4转录促进鸡雄性分化。我们的研究结果为鸡雄性分化的分子机制提供了新的见解。
SPINDLIN1-Z (SPIN1Z), a member of the Spin/Ssty(Y-linked spermiogenesis specific transcript) protein family, participates in the early embryonic development process. Our previous RNA-seq analysis indicates that the level of Spin1z was abundantly expressed in male embryonic stem cells (ESCs) and primitive germ cells (PGCs), we speculate that Spin1z may play an important role in chicken male differentiation. Therefore, the loss-and gain-of-function experiments provide solid evidence that Spin1z is both necessary and sufficient to initiate male development in chicken. Furthermore, chromatin immunoprecipitation (ChIP) assay and the dual-luciferase assay was performed to further confirm that Spin1z contributed to chicken male differentiation by inhibiting the Tcf4 transcription. Our findings provide a novel insight into the molecular mechanism for chicken male differentiation.