Magnetic resonance imaging of mass transport and structure inside a phototrophic biofilm.
Magnetic resonance imaging of mass transport and structure inside a phototrophic biofilm.
复制标题
光养生物膜内的质量传输和结构的磁共振成像。
DOI：
10.1007/s00284-012-0292-3
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发表时间：
2013
影响因子：
2.6
通讯作者：
Ramanan B
中科院分区：
文献类型：
--
作者：
Ramanan B
本研究的目的是利用磁共振成像（MRI）成像的结构异质性和质量运输内的生物膜是太厚的光子成像。MRI被用来映射水的扩散和图像的顺磁性标记的大分子，Gd-DTPA，内2.5毫米厚的蓝藻生物膜的运输。生物膜的结构异质性成像分辨率低至22 × 22 μm，使生物膜结构对水和Gd-DTPA的质量传输的影响进行研究。生物膜的密度较高的地区与表现出较低的相对水扩散系数和较慢的Gd-DTPA运输的地区，突出了生物膜结构对传质现象的影响。这种方法有可能揭示一系列分子质量分子在生物膜中的异质质量传输。
The aim of this study was to utilize magnetic resonance imaging (MRI) to image structural heterogeneity and mass transport inside a biofilm which was too thick for photon based imaging. MRI was used to map water diffusion and image the transport of the paramagnetically tagged macromolecule, Gd-DTPA, inside a 2.5 mm thick cyanobacterial biofilm. The structural heterogeneity of the biofilm was imaged at resolutions down to 22 × 22 μm, enabling the impact of biofilm architecture on the mass transport of both water and Gd-DTPA to be investigated. Higher density areas of the biofilm correlated with areas exhibiting lower relative water diffusion coefficients and slower transport of Gd-DTPA, highlighting the impact of biofilm structure on mass transport phenomena. This approach has potential for shedding light on heterogeneous mass transport of a range of molecular mass molecules in biofilms.