Dielectric barrier discharge micro-plasma emission spectrometry for the detection of acetone in exhaled breath
Dielectric barrier discharge micro-plasma emission spectrometry for the detection of acetone in exhaled breath
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介质阻挡放电微等离子体发射光谱法检测呼出气中的丙酮
DOI：
10.1016/j.talanta.2015.07.074
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发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
Wang Jian-Hua
中科院分区：
文献类型：
--
作者：
Yang Ting;Gao Dong-Xue;Yu Yong-Liang;Chen Ming-Li;Wang Jian-Hua
丙酮是呼出气中的主要挥发性有机化合物（VOC），也是糖尿病和酮症酸中毒的有前景的生物标志物。在平面介质阻挡放电（DBD）中产生的非热微等离子体被用作激发气态丙酮的辐射源，随后用光学发射光谱（OES）对其进行定量。气态丙酮可直接进样，液态丙酮经加热钨线圈蒸发后，通过氦载流引入DBD微等离子体中，在519 nm发射线处进行光发射和检测。在本研究中，收集呼出气并转移到水性介质中进行采样。方法的线性范围为沿着增大，线性范围为40-1600 mg L− 1，检出限为44 ng，相对标准偏差为5.7%。本系统成功地应用于糖尿病患者和健康志愿者呼吸丙酮的测定。
Acetone is a predominant volatile organic compound (VOC) in the exhaled breath and a promising biomarker for diabetes and ketoacidosis. A non-thermal micro-plasma generated in a planar dielectric barrier discharge (DBD) is used as a radiation source for the excitation of gaseous acetone followed by its quantification with optical emission spectrometry (OES). Gaseous acetone can be directly sampled, while liquid acetone is evaporated by heated tungsten coil and then introduced into the DBD micro-plasma by a helium carrier flow for performing optical emission and detection at a 519 nm emission line. In the present study, the exhaled breath is collected and transferred into aqueous medium for sampling. With a sampling volume of 7 μL in a micro-drop, a linear range of 40–1600 mg L−1is obtained along with a detection limit of 44 ng and a precision of 5.7% RSD. The present system is successfully applied to the determination of breath acetone for both diabetic patients and healthy volunteers.