Transmembrane channel-like (tmc) gene regulates Drosophila larval locomotion
Transmembrane channel-like (tmc) gene regulates Drosophila larval locomotion
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DOI：
10.1073/pnas.1606537113
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发表时间：
2016-06-28
影响因子：
11.1
通讯作者：
Wang, Zuoren
中科院分区：
文献类型：
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作者：
Guo, Yanmeng;Wang, Yuping;Wang, Zuoren
果蝇幼虫的运动，这需要有节奏的身体收缩，是由本体感受器的感觉反馈控制。介导这种反馈的分子机制知之甚少。通过基因敲入和免疫染色，我们发现果蝇跨膜通道样（TMC）基因表达的幼虫I类和II类树突状分支（DA）神经元和双极树突（BD）神经元，这两者都是已知的幼虫运动提供感觉反馈。被击倒或失去tmc功能的幼虫表现出爬行速度降低，头部投掷频率增加，向后运动增强。在tmc阳性神经元中表达果蝇TMC或哺乳动物TMC 1和/或TMC 2拯救了这些突变表型。幼虫身体的弯曲激活了tmc阳性神经元，而在tmc突变体中，这种弯曲反应被削弱。这暗示TMC在果蝇本体感受和幼虫运动的感觉控制中的作用。这也为果蝇和哺乳动物TMCs之间的功能保守性提供了证据。
Drosophila larval locomotion, which entails rhythmic body contractions, is controlled by sensory feedback from proprioceptors. The molecular mechanisms mediating this feedback are little understood. By using genetic knock-in and immunostaining, we found that the Drosophila melanogaster transmembrane channel-like (tmc) gene is expressed in the larval class I and class II dendritic arborization (da) neurons and bipolar dendrite (bd) neurons, both of which are known to provide sensory feedback for larval locomotion. Larvae with knockdown or loss of tmc function displayed reduced crawling speeds, increased head cast frequencies, and enhanced backward locomotion. Expressing Drosophila TMC or mammalian TMC1 and/or TMC2 in the tmc-positive neurons rescued these mutant phenotypes. Bending of the larval body activated the tmc-positive neurons, and in tmc mutants this bending response was impaired. This implicates TMC's roles in Drosophila proprioception and the sensory control of larval locomotion. It also provides evidence for a functional conservation between Drosophila and mammalian TMCs.