Cation-exchange high-performance liquid chromatography assay for the nigrostriatal toxicant 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and its monoamine oxidase B generated metabolites in brain tissues.
Cation-exchange high-performance liquid chromatography assay for the nigrostriatal toxicant 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and its monoamine oxidase B generated metabolites in brain tissues.
复制标题
阳离子交换高效液相色谱法测定脑组织中黑质纹状体毒物1-甲基-4-苯基-1,2,3,6-四氢吡啶及其单胺氧化酶B产生的代谢物。
DOI：
10.1016/s0021-9673(01)96513-6
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Trevor,AJ
中科院分区：
文献类型：
--
作者：
Shinka,T;CastagnoliJr,N;Wu,EY;Hoag,MK;Trevor,AJ
本文介绍了一种灵敏（1 pmol/mg组织）和选择性的黑纹状体毒物1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）及其单胺氧化酶B产生的代谢物，1-甲基-4-苯基-2,3-二氢吡啶种MPDP+和1-甲基-4-苯基吡啶种MPP+的定量分析方法。该方法基于脑组织匀浆用5%三氯乙酸处理后分析物的初始分离。采用二极管阵列紫外检测器直接用阳离子交换高效液相色谱法分析可溶性组分。通过该实验获得的结果为静脉注射MPTP后小鼠大脑中MPDP+的存在提供了第一个证据。
This paper describes a sensitive (1 pmol/mg tissue) and selective bioanalytical method for the quantitative estimation of the nigrostriatal toxicant 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its monoamine oxidase B generated metabolites, the 1-methyl-4-phenyl-2,3-dihydropyridium species MPDP+and the 1-methyl-4-phenylpyridinium species MPP+. The method is based on initial separation of the analytes after treatment of brain tissue homogenates with 5% trichloroacetic acid. The soluble fraction is analyzed directly by cation-exchange high-performance liquid chromatography employing a diode array UV detector. Results obtained with this assay have provided the first evidence for the presence of MPDP+in the mouse brain following intravenous administration of MPTP.