Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue
Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue
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DOI：
10.1073/pnas.1314447111
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发表时间：
2014-04-08
影响因子：
11.1
通讯作者：
Patterson, George H.
中科院分区：
文献类型：
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作者：
Ingaramo, Maria;York, Andrew G.;Patterson, George H.
多焦结构照明显微镜（MSIM）提供了一个两倍的分辨率增强超过衍射极限，在样品深度高达50 μ m，但散射和离焦的光在厚样品降低MSIM性能。在这里，我们实现MSIM与微透镜阵列，使有效的双光子激发。双光子MSIM提供分辨率加倍的图像，在厚散射样品中具有更好的切片和对比度，例如秀丽隐杆线虫胚胎，果蝇幼虫唾液腺和小鼠肝脏组织。
Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 mu m, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.