Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction.
Transmission of Chlamydia trachomatis among sex partners assessed by polymerase chain reaction.
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通过聚合酶链反应评估沙眼衣原体在性伴侣之间的传播。
DOI：
10.1093/infdis/168.2.488
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Quinn,TC
中科院分区：
文献类型：
--
作者：
Viscidi,RP;Bobo,L;Hook3rd,EW;Quinn,TC
用聚合酶链反应（PCR）和细胞培养法检测了95例性伴衣原体感染情况。186份标本中33份（18%）培养阳性，61份（33%）PCR-EIA阳性。PCR阳性女性的男性伴侣中有75%（21/28）的PCR阳性，而培养阳性女性的男性伴侣中只有45%（9/19）的PCR阳性（P= 0.053）。对于受感染男性的女性伴侣，差异不太明显。感染者的女性伴侣PCR阳性率为58%（21/36），培养阳性者的女性伴侣PCR阳性率为56%（15/36）。7对夫妇性伴侣的衣原体DNA序列分析与性伴侣间的相关性支持了该方法的准确性。这些数据表明，PCR比培养更敏感，尤其是对感染妇女的男性伴侣。
The prevalence ofChlamydiainfection in 95 sex partners was determined by both polymerase chain reaction (PCR) and cell culture. Thirty-three (18%) of 186 specimens were positive by culture and 61 (33%) were positive by PCR-EIA. PCR was positive in 75% (21/28) of male partners of PCR-positive women compared with culture, which was positive in only 45% (9/19) of male partners of culture-positive women (P= .053). For female partners of infected men, the difference was less marked. PCR was positive in 58% (21/36) of female partners of infected men versus culture, which was positive in 56% (15/36) offemale partners of culture-positive men. The correlation of PCR between partners and sequence analysis ofChlamydiaDNA showing the same sequence from sex partners of 7 couples support the accuracy of the assay. These data suggest that PCR is more sensitive than culture for detection ofChlamydia trachomatis, particularly for male partners of infected women.