MSC Frequency Correlates with Blood Vessel Density in Equine Adipose Tissue
MSC Frequency Correlates with Blood Vessel Density in Equine Adipose Tissue
复制标题
DOI：
10.1089/ten.tea.2008.0103
复制
发表时间：
2009-02-01
影响因子：
4.1
通讯作者：
Caplan, Arnold I.
中科院分区：
文献类型：
--
作者：
Meirelles, Lindolfo da Silva;Sand, Theodore T.;Caplan, Arnold I.
间充质干细胞（MSCs）是多能细胞，具有分化为不同成熟间充质细胞类型的能力。它们最初是从骨髓中分离出来的，但类似间充质干细胞的细胞也已从其他组织中分离出来。所有这些组织的共同特征是它们都有血管。因此，间充质干细胞有可能与血管周围位置相关。这项工作的目的是通过验证间充质干细胞的频率是否与血管密度正相关来检验间充质干细胞与血管相关的这一假设。为此，收集了来自两匹马供体的血管丰富和血管稀少的脂肪组织部位的样本，并进行组织学和细胞分离处理。通过在间充质干细胞条件下进行的集落形成单位 - 成纤维细胞（CFU - F）测定来估计这些样本中间充质干细胞的频率。对来自马脂肪组织和骨髓的贴壁培养细胞进行培养扩增，在体外测试其向间充质细胞类型的分化能力，并将其植入多孔陶瓷载体在体内评估其成骨能力，同时以人间充质干细胞和脑周细胞作为对照。分化测定显示，与马骨髓间充质干细胞相比，脂肪组织来源的细胞之间存在差异。虽然两位供体之间的集落形成单位 - 成纤维细胞频率差异明显，但集落形成单位 - 成纤维细胞数量与血管密度直接相关（r² = 0.86）。我们认为这些初步数据是将间充质干细胞与血管联系起来的进一步证据。
Mesenchymal stem cells (MSCs) are multipotent cells that have the capacity to develop into different mature mesenchymal cell types. They were originally isolated from bone marrow, but MSC-like cells have also been isolated from other tissues. The common feature of all of these tissues is that they all house blood vessels. It is, thus, possible that MSCs are associated with perivascular locations. The objective of this work was to test the hypothesis that MSCs are associated with blood vessels by verifying if MSC frequency positively correlates with blood vessel density. To this end, samples from highly and poorly vascularized adipose tissue sites of two equine donors were collected and processed for histology and cell isolation. MSC frequency in these samples was estimated by means of CFU-F assays, which were performed under MSC conditions. Culture-adherent cells from equine adipose tissue and bone marrow were culture expanded, tested for differentiation into mesenchymal cell types in vitro, and implanted in vivo in porous ceramic vehicles to assess their osteogenic capacity, using human MSCs and brain pericytes as controls. The differentiation assays showed a difference between adipose tissue derived cells as compared to equine bone marrow MSCs. While differences in CFU-F frequencies between both donors were evident, the CFU-F numbers correlated directly with blood vessel densities (r(2)= 0.86). We consider these preliminary data as further evidence linking MSCs to blood vessels.