Improving the success of culturing Helicobacter pylori from gastric biopsies.
Improving the success of culturing Helicobacter pylori from gastric biopsies.
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提高胃活检培养幽门螺杆菌的成功率。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
B. Ho
中科院分区：
文献类型：
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作者：
J. Hua;K. Yeoh;H. Ng;P. Zheng;S. Lim;B. Ho
研究了影响从人胃活检中成功分离幽门螺杆菌的因素。 24 小时内，将每个胃活检组织接种到巧克力血琼脂培养基上并孵育长达 2 周。在 90 名患者的 63 例（70%）培养阳性病例中，58 例（64%）两份标本均呈培养阳性，而 5 例（6%）例仅在一份活检中培养阳性。在 63 个阳性培养物中，51 个幽门螺杆菌菌株 (81%) 在含有和不含抗生素的培养基上均生长。八个菌株（13%）仅在不含抗生素的培养基上生长，而四个分离株（6%）仅从含有抗生素的培养基中获得。这些结果支持了先前对胃中幽门螺杆菌斑片定植的组织学观察。当采集两次活组织检查并将其接种到含有或不含有抗生素的巧克力血琼脂培养基上时，从胃活检中培养幽门螺杆菌的成功率会增加。
Factors influencing the successful isolation of Helicobacter pylori from human gastric biopsies were studied. Within 24 h, each of the gastric biopsies was inoculated onto chocolate blood agar media and incubated for up to 2 weeks. Among 63 (70%) culture positive cases in 90 patients, 58 (64%) cases were culture positive for both specimens, while five (6%) cases were culture positive in only one biopsy. Of the 63 positive cultures, 51 H. pylori strains (81%) grew on both media with and without antibiotics. Eight strains (13%) grew only on medium without antibiotics, while four isolates (6%) were obtained only from medium with antibiotics. These results support the previous histological observation of patchy colonization of H. pylori in the stomach. The success rate for culture of H. pylori from gastric biopsies increased when two biopsies were taken and inoculated on chocolate blood agar media with and without antibiotics.