Advances in Microsphere-based Super-resolution Imaging
Advances in Microsphere-based Super-resolution Imaging
复制标题
基于微球的超分辨率成像的进展
DOI：
10.1109/rbme.2024.3355875
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发表时间：
2024
影响因子：
17.6
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Upreti, Neil;Jin, Geonsoo;Rich, Joseph;Zhong, Ruoyu;Mai, John;Zhao, Chenglong;Huang, Tony Jun
在细胞和分子生物学、生物物理学和纳米技术等各个领域，纳米级分辨率对于理解大规模分子相互作用的复杂细节至关重要，因此有必要使用大视场（FOV）来解析超出光衍射极限的图像。虽然存在实现超分辨率的几种方法，但它们通常受到诸如高成本、显著复杂性、冗长的处理时间以及图像分辨率和FOV之间的经典权衡等因素的阻碍。基于微球的超分辨率成像已经成为解决这些限制的有前途的方法。在这篇综述中，我们深入研究了基于微球的成像和相关的光子纳米喷射的理论基础。其次是各种基于微球的成像技术的全面探索，包括静态成像，机械扫描，光学扫描和声流体扫描方法。本文最后对这一创新技术的潜在应用和未来的科学方向进行了前瞻性的展望。
Techniques to resolve images beyond the diffraction limit of light with a large field of view (FOV) are necessary to foster progress in various fields such as cell and molecular biology, biophysics, and nanotechnology, where nanoscale resolution is crucial for understanding the intricate details of large-scale molecular interactions. Although several means of achieving super-resolutions exist, they are often hindered by factors such as high costs, significant complexity, lengthy processing times, and the classical tradeoff between image resolution and FOV. Microsphere-based super-resolution imaging has emerged as a promising approach to address these limitations. In this review, we delve into the theoretical underpinnings of microsphere-based imaging and the associated photonic nanojet. This is followed by a comprehensive exploration of various microsphere-based imaging techniques, encompassing static imaging, mechanical scanning, optical scanning, and acoustofluidic scanning methodologies. This review concludes with a forward-looking perspective on the potential applications and future scientific directions of this innovative technology.