Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase.
Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase.
复制标题
具有同构核碱基的 SAM 类似物的化学酶合成和利用。
DOI：
10.1039/c6ob00844e
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发表时间：
2016
影响因子：
3.2
通讯作者：
Tor,Y
中科院分区：
文献类型：
--
作者：
Vranken,C;Fin,A;Tufar,P;Hofkens,J;Burkart,MD;Tor,Y
萨尔是一种催化L-甲硫氨酸和5′-氯-5 ′-脱氧腺苷合成SAM的酶，它能接受5′-氯-5 ′-脱氧噻吩腺苷作为底物，并促进合成具有非天然核碱基的SAM类似物。证明该合成辅因子在与M. TaqI的DNA甲基化反应中替代SAM。
SalL, an enzyme that catalyzes the synthesis of SAM from L-methionine and 5′-chloro-5′-deoxyoadenosine, is shown to accept 5′-chloro-5′-deoxythienoadenosine as a substrate and facilitate the synthesis of a synthetic SAM analog with an unnatural nucleobase. This synthetic cofactor is demonstrated to replace SAM in the DNA methylation reaction with M.TaqI.