Astrocyte-like glia associated with the embryonic development of the central complex in the grasshopper Schistocerca gregaria
Astrocyte-like glia associated with the embryonic development of the central complex in the grasshopper Schistocerca gregaria
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DOI：
10.1007/s00427-011-0366-4
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发表时间：
2011-08-01
影响因子：
2.4
通讯作者：
Liu, Yu
中科院分区：
文献类型：
--
作者：
Boyan, George;Loser, Michael;Liu, Yu
在这项研究中，除了神经胶质细胞特异性标记物 Repo 之外，我们还利用星形胶质细胞特异性酶谷氨酰胺合成酶的表达来表征与蚱蜢 Schistocerca gregaria 中央复合体胚胎发育相关的神经胶质细胞类型。双标记实验表明，所有与中心复合物相关的谷氨酰胺合成酶阳性细胞也是 Repo 阳性和辣根过氧化物酶阴性，证实它们是神经胶质细胞。在胚胎发生的早期，在中央复合体发育之前，神经胶质细胞形成从脑间部延伸到连合束区域的连续群体。随后，这些神经胶质细胞重新分散，包围中央复合体的每个模块。在中央复合体神经纤维本身内没有发现神经胶质体。由于谷氨酰胺合成酶在神经胶质细胞的皮质中表达，因此可以使它们的过程及其胞体位置可视化。单细胞重建揭示了一组神经胶质细胞指导着中央复杂神经纤维（例如中央体）周围的广泛鞘化过程，而另一组则在中央体内投射出柱状树状结构。这种树枝状结构仅在其神经结构建立后才出现在中央复杂模块中，这表明神经胶质树枝状结构投射到先前的神经元或气管支架上。
In this study we employed the expression of the astrocyte-specific enzyme glutamine synthetase, in addition to the glia-specific marker Repo, to characterize glia cell types associated with the embryonic development of the central complex in the grasshopper Schistocerca gregaria. Double labeling experiments reveal that all glutamine synthetase-positive cells associated with the central complex are also Repo-positive and horseradish peroxidase-negative, confirming they are glia. Early in embryogenesis, prior to development of the central complex, glia form a continuous population extending from the pars intercerebralis into the region of the commissural fascicles. Subsequently, these glia redisperse to envelop each of the modules of the central complex. No glial somata are found within the central complex neuropils themselves. Since glutamine synthetase is expressed cortically in glia, it allows their processes as well as their soma locations to be visualized. Single cell reconstructions reveal one population of glia as directing extensive ensheathing processes around central complex neuropils such as the central body, while another population projects columnar-like arborizations within the central body. Such arborizations are only seen in central complex modules after their neuroarchitecture has been established suggesting that the glial arborizations project onto a prior scaffold of neurons or tracheae.