No evidence for mitochondrial genetic variability in the largest population of critically endangered Tonkin snub-nosed monkeys in Vietnam
No evidence for mitochondrial genetic variability in the largest population of critically endangered Tonkin snub-nosed monkeys in Vietnam
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DOI：
10.1007/s10329-016-0571-x
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发表时间：
2016-10-01
期刊：
影响因子：
1.7
通讯作者：
Covert, Herbert
中科院分区：
文献类型：
--
作者：
Ang, Andie;Srivathsan, Amrita;Covert, Herbert
东京金丝猴（学名：Rhinopithecus avunculus），全球种群数量少于250只，被列为极度濒危物种。它是越南东北部的特有种，在1989年被重新发现之前，人们一直担心它已经灭绝。最大的单一种群R. avunculus由125-130个个体组成，位于河江省的Khau Ca森林地区。我们使用非侵入性收集的粪便样本，根据线粒体信息确定该人群的遗传多样性。我们扩增并测序了201个样本的线粒体D环高变区I（HVI）的467- 650-bp部分，并基于宏基因组数据重建了5个样本的完整线粒体基因组。所有201个HVI序列都是相同的，在5个线粒体基因组中没有发现变异。我们的研究结果强调，迫切需要全面评估所有种群的遗传多样性的R。Avunculus基于线粒体和核标记。后者需要为这个物种开发。
The Tonkin snub-nosed monkey (Rhinopithecus avunculus) with a global population of < 250 is listed as critically endangered. It is endemic to northeastern Vietnam and was feared extinct until its rediscovery in 1989. The largest single population of R. avunculus consists of 125-130 individuals in an area of forest called Khau Ca in Ha Giang Province. We used non-invasively collected fecal samples to establish the amount of genetic diversity in this population based on mitochondrial information. We amplified and sequenced a 467- to 650-bp section of the hypervariable region I (HVI) of the mitochondrial D-loop for 201 samples and reconstructed the full mitochondrial genomes for five samples based on metagenomic data. All 201 HVI sequences were identical and no variability was found in the five mitochondrial genomes. Our results highlight the immediate need for a comprehensive assessment of the genetic diversity of all populations of R. avunculus based on mitochondrial and nuclear markers. The latter need to be developed for this species.