Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study
Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study
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DOI：
10.1007/s13277-015-4164-x
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发表时间：
2016-04
期刊：
影响因子：
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通讯作者：
Mingzhu Yin;S. Tan;Xia Li;Y. Hou;Guosheng Cao;Kang Li;J. Kou;G. Lou
中科院分区：
文献类型：
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作者：
Mingzhu Yin;S. Tan;Xia Li;Y. Hou;Guosheng Cao;Kang Li;J. Kou;G. Lou
代谢产物是细胞调节过程的终产物。宫颈鳞癌（Squamous cervical cancer，SCC）可通过调节基因表达改变血浆中某些小分子代谢产物的水平。在这项研究中，我们确定了两种代谢产物，磷脂酰胆碱（PC）和溶血磷脂酰胆碱（LPC），这是显着下调和上调的血浆中SCC相比，子宫肌瘤（UF）患者通过超高效液相色谱-质谱（UPLC-MS）。在外部前瞻性队列中，我们的检测方法的灵敏度为93.2%，特异性为91.3%，受试者工作特征（ROC）曲线下面积（AUC）为0.972。SCC患者LPC水平明显高于UF患者。在PC水平观察到相反的结果。我们的研究结果表明，PC和lysoPC可以作为新的生物标志物，以促进SCC的诊断。
Metabolites are the end products of cellular regulatory processes. Squamous cervical cancer (SCC) can alter the level of certain small molecular metabolite in plasma through modulating gene expression. In this study, we identified two metabolites, phosphatidylcholine (PC) and lysophosphatidylcholine (LPC), which are significantly down- and upregulated in plasma of SCC as compared to uterine fibroid (UF) patients via ultra-performance liquid chromatographic-mass spectrometry (UPLC-MS). In external prospective cohort, our assay has a sensitivity of 93.2 %, a specificity of 91.3 %, and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.972. The level of LPC is significantly higher in SCC than in UF patients. An opposite result was observed in PC level. Our findings suggest that the PC and lysoPC could be used as novel biomarkers to facilitate SCC diagnosis.