DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE
DIRECT CORRELATION OF PARVALBUMIN LEVELS WITH MYOSIN ISOFORMS AND SUCCINATE-DEHYDROGENASE ACTIVITY ON FROZEN-SECTIONS OF RODENT MUSCLE
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DOI：
10.1177/39.3.1825216
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发表时间：
1991-03-01
影响因子：
3.2
通讯作者：
JOCKUSCH, H
中科院分区：
文献类型：
--
作者：
FUCHTBAUER, EM;ROWLERSON, AM;JOCKUSCH, H
小清蛋白（PV）是一种可溶性钙离子结合蛋白，在啮齿类动物的快肌中尤为集中。 我们已经开发了一种新的协议，以固定冷冻切片的肌肉甲醛蒸汽，这使我们能够免疫化学染色连续冷冻切片PV。 纤维类型的定义是基于肌球蛋白ATP酶的稳定性，以及由各种抗体鉴定的异肌球蛋白，因为ATP酶的稳定性单独在小鼠中产生不明确的结果。 小鼠和大鼠的慢I型纤维缺乏PV，并且具有中等至高SDH水平。 在小鼠中，根据其肌球蛋白重链免疫反应性与大鼠中这些类型的相似性，定义了快纤维亚型IIA、IIB和IIX样。 比目鱼肌通常是PV阴性，但在小鼠中存在少量强烈PV阳性的IIX样纤维。 在小鼠快肌中，小直径IIA纤维呈PV阴性，SDH活性高。 在小鼠和大鼠中，IIB和IIX纤维的PV反应性高于IIA和I，而IIA、IIX和I纤维的SDH水平高于IIB。 因此，PV含量与肌球蛋白ATP酶的类型相关，但与SDH水平无关。 PV的免疫细胞化学所描述的方法可能适用于其他高可溶性蛋白。
Parvalbumin (PV) is a soluble Ca++ binding protein which is particularly concentrated in fast muscles of rodents. We have developed a new protocol to fix frozen sections of muscle by formaldehyde vapor, which enabled us to immunochemically stain serial frozen sections for PV. Fiber types were defined on the basis of myosin ATPase stability, and of isomyosins identified by a variety of antibodies because ATPase stability alone yielded ambiguous results in the mouse. Slow Type I fibers in mouse and rat were devoid of PV and had intermediate to high SDH levels. Fast fiber subtypes IIA, IIB, and IIX-like were defined in the mouse on the basis of the similarity of their myosin heavy chain immunoreactivity to these types in the rat. The soleus muscle was usually PV negative, but a small population of strongly PV-positive IIX-like fibers was present in the mouse. In mouse fast muscle, small diameter IIA fibers were PV negative with high SDH activity. In both mouse and rat, PV reactivities of IIB and IIX fibers were higher than those of IIA and I, whereas SDH levels of IIA, IIX, and I fibers were higher than those of IIB. Thus, PV content correlated with the type of myosin ATPase but not with SDH levels. The method described for immunocytochemistry of PV may be applicable to other highly soluble proteins.