Cytocompatible Polymer Grafting from Individual Living Cells by Atom-Transfer Radical Polymerization
Cytocompatible Polymer Grafting from Individual Living Cells by Atom-Transfer Radical Polymerization
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DOI：
10.1002/anie.201608515
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发表时间：
2016-12-05
影响因子：
16.6
通讯作者：
Choi, Insung S.
中科院分区：
文献类型：
--
作者：
Kim, Ji Yup;Lee, Bong Soo;Choi, Insung S.
一种细胞相容的方法，表面引发，活化剂再生的电子转移，原子转移自由基聚合（SI-ARGET ATRP）的开发工程细胞表面与合成聚合物。基于多巴胺的ATRP引发剂用于将ATRP引发剂均匀地引入到化学复杂的细胞表面上（通过聚多巴胺的材料独立的涂层性质）和保护细胞在聚合期间免受自由基攻击（通过聚多巴胺的自由基清除性质）。合成的聚合物接枝到单个酵母细胞的表面上，而没有显著的细胞活力损失，并且通过各种表征方法（包括凝集测定和细胞分裂研究）证实了均匀和致密的接枝。这项工作将为活细胞-聚合物混合结构的产生提供一种战略方法，并为其在传感器技术、催化、治疗诊断学和细胞治疗等众多领域的应用打开大门。
A cytocompatible method of surface-initiated, activator regenerated by electron transfer, atom transfer radical polymerization (SI-ARGET ATRP) is developed for engineering cell surfaces with synthetic polymers. Dopamine-based ATRP initiators are used for both introducing the ATRP initiator onto chemically complex cell surfaces uniformly (by the material-independent coating property of polydopamine) and protecting the cells from radical attack during polymerization (by the radical-scavenging property of polydopamine). Synthetic polymers are grafted onto the surface of individual yeast cells without significant loss of cell viability, and the uniform and dense grafting is confirmed by various characterization methods including agglutination assay and cell-division studies. This work will provide a strategic approach to the generation of living cell-polymer hybrid structures and open the door to their application in multitude of areas, such as sensor technology, catalysis, theranostics, and cell therapy.