Widespread distribution of a chorionic gonadotropin-like substance in normal human tissues.
Widespread distribution of a chorionic gonadotropin-like substance in normal human tissues.
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正常人体组织中广泛分布绒毛膜促性腺激素样物质。
DOI：
10.1210/jcem-49-6-917
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
Maclyn E. Wade
中科院分区：
文献类型：
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作者：
Braunstein Braunstein;Vikram A Kamdar;J. Rasor;N. Swaminathan;Maclyn E. Wade
最近的研究表明，正常人的睾丸、结肠和肝脏中含有一种类似hCG的物质。为了扩展这些发现，我们检查了各种正常人体组织的水提取物中是否存在这种材料。β-hCG RIA，大鼠Leydig细胞放射受体测定，和一个新开发的，高度特异性的hCG RIA被用来测量hCG活性的一系列稀释的提取物。在研究的149个个体组织样本中，有146个发现了可检测浓度的hCG样物质。在β-hCG RIA、放射受体测定和高特异性hCG RIA中，hCG标准品与睾丸、卵巢、垂体、肺、肝、肾、脾、胃、胎盘和一些小肠组织样品的提取物的系列稀释液之间观察到了亲和性。在β-LH RIA中，发现非垂体组织提取物与LH之间不存在平行关系。胰腺提取物改变了在这些测定中用作标记配体的[125 I]hCG，从而导致假结果。提取物在ConcanavalinA-Sepharose柱上的层析显示，来自不同组织的hCG样物质在其对ConcanavalinA的吸附上差异很大，这可能反映了它们的碳水化合物含量的差异。这些结果表明，hCG样物质广泛分布在整个正常人体组织，并进一步支持的概念，负责hCG生产的胎儿基因组在成人组织中没有完全抑制。
Recent studies have demonstrated that the normal human testes, colon, and liver contain a substance that resembles hCG. To extend these findings, we examined aqueous extracts of a variety of normal human tissues for the presence of this material. The beta-hCG RIA, rat Leydig cell radioreceptor assay, and a newly developed, highly specific hCG RIA were used to measure hCG activity in a serial dilutions of the extracts. Detectable concentrations of the hCG-like material were found in 146 of the 149 individual tissue samples studied. Parallelism was noted between the hCG standard and serial dilutions of extracts of testis, ovary, pituitary, lung, liver, kidney, spleen, stomach, placenta, and some small intestinal tissue samples in the beta-hCG RIA, radioreceptor assay, and the highly specific hCG RIA. An absence of parallelism was found between extracts of nonpituitary tissues and LH in the beta-LH RIA. Pancreatic extracts altered the [125I]hCG used as the labeled ligand in these assays, which led to spurious results. Chromatography of the extracts on Concanavalin A-Sepharose columns revealed that the hCG-like materials from different tissues varied widely in their adsorbtion to Concanavalin A, possibly reflecting differences in their carbohydrate contents. These results indicate that an hCG-like substance is widely distributed throughout normal human tissues and further supports the concept that the fetal genome responsible for hCG production is not completely suppressed in adult tissues.