Roseomonas rhizosphaerae sp nov., a triazophosdegrading bacterium isolated from soil
Roseomonas rhizosphaerae sp nov., a triazophosdegrading bacterium isolated from soil
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Roseomonas rhizosphaerae sp nov.，一种从土壤中分离的三唑磷降解细菌
DOI：
10.1099/ijs.0.057000-0
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发表时间：
2014-04-01
影响因子：
2.8
通讯作者：
Gu, Jin-gang
中科院分区：
文献类型：
--
作者：
Chen, Qing;Sun, Li-Na;Gu, Jin-gang
从长期施用三唑磷的土壤中分离出一株新型需氧、不形成芽孢、无运动性、过氧化氢酶和氧化酶阳性、革兰氏染色阴性、球状至短杆状的细菌菌株，命名为YW11(T)。该菌株能够水解三唑磷。菌株YW11(T)在15 - 40℃（最适温度为28℃）、pH 5.0 - 8.0（最适pH为7.5）以及含0 - 5.0%（w/v）NaCl（最适为0.5%）的条件下生长。主要呼吸醌为泛醌 - 10（Q - 10），主要细胞脂肪酸为C - 18:1ω7c、C - 16:0、C - 18:1 2 - OH和C - 18:0。菌株YW11(T)的基因组DNA G + C含量为69.6 ± 0.5 mol%。主要极性脂质为磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰胆碱、一种未知糖脂和两种未知氨基脂。基于16S rRNA基因序列比较的系统发育分析表明，菌株YW11(T)是玫瑰单胞菌属（Roseomonas）的成员，与颈玫瑰单胞菌（Roseomonas cervicalis）KACC 11686(T)（97.9%）和河口玫瑰单胞菌（Roseomonas aestuaril）KACC 19645(T)（97.8%）的序列相似性最高，其次是卢迪普玫瑰单胞菌（Roseomonas ludipueritiae）KACC 138431（96.9%）。菌株YW11(T)与颈玫瑰单胞菌KACC 116861（32.3 ± 2.9%）、河口玫瑰单胞菌KACC 16549(T)（28.2 ± 2.6%）和卢迪普玫瑰单胞菌KACC 138431（30.2 ± 2.6%）的DNA - DNA相关性较低。基于系统发育分析和DNA - DNA杂交结果、全细胞脂肪酸组成以及生化特性，菌株YW11(T)明显区别于玫瑰单胞菌属的所有已确认物种，应被归为玫瑰单胞菌属的一个新种，提议将其命名为根际玫瑰单胞菌（Roseomonas rhizosphaerae sp. nov.）。模式菌株为YW11(T)（=KACC 17225(T)=CCTCC AB2013041(T)）
A novel aerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-stain-negative, coccoid to short-rod-shaped bacterial strain, designated YW11(T), was isolated from soil under long-term application of triazophos. The strain was able to hydrolyse triazophos. Strain YW11(T) grew at 15-40 degrees C (optimum at 28 degrees C), at pH 5.0-8.0 (optimum at pH 7.5) and with 0-5.0 % (w/v) NaCl (optimum at 0.5 %). The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were C-18:1 omega 7c, C-16:0, C-18:1 2-OH and C-18:0. The genomic DNA G+C content of strain YW11T was 69.6 +/- 0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unknown glycolipid and two unknown aminolipids. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain YW11(T) was a member of the genus Roseomonas, and showed the highest sequence similarity to Roseomonas cervicalis KACC 11686(T) (97.9 %) and Roseomonas aestuaril KACC 19645(T) (97.8%) and then to Roseomonas ludipueritiae KACC 138431 (96.9%). Strain YW11(T). showed low DNA DNA relatedness with R. cervicalis KACC 116861 (32.3 2.9%), R. aestuarii KACC 16549(T) (28.2 2.6%) and R. ludipueritiae KACC 138431 (30.2 2.6 %). Based on the results of phylogenetic analysis and DNA DNA hybridization, the whole-cell fatty acid composition as well as biochemical characteristics, strain YW11(T) was clearly distinguished from all recognized species of the genus Roseomonas and should be assigned to a novel species of the genus Roseomonas, for which the name Roseomonas rhizosphaerae sp. nov. is proposed. The type strain is YVV11(T) (=KACC 17225(T)=CCTCC AB2013041(T)).