MONOCYTE-MEDIATED ERYTHROCYTE DESTRUCTION - A COMPARATIVE-STUDY OF CURRENT METHODS
MONOCYTE-MEDIATED ERYTHROCYTE DESTRUCTION - A COMPARATIVE-STUDY OF CURRENT METHODS
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DOI：
10.1046/j.1537-2995.1981.21682085766.x
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
WOOD, GW
中科院分区：
文献类型：
--
作者：
HUNT, JS;BECK, ML;WOOD, GW
采用EAIgG[红细胞、抗体、IgG]花环形成、51Cr释放和红细胞吞噬三种检测系统，定量测定抗体包被的人红细胞与正常血液单核细胞之间的相互作用。比较3种方法的时间要求和灵敏度。红细胞吞噬比玫瑰花结试验（30分钟）需要更多的时间（2小时），但比51Cr释放试验（5.5小时）要少。红细胞吞噬术的敏感性是其他两种方法的20倍。纯化的IgG抗d抗体与输血或妊娠诱导的抗体结果相似。
Three assay systems, EAIgG [erythrocyte, antibody, IgG] rosette formation, 51Cr release and erythrophagocytosis, were used to quantitate interaction between antibody-coated human erythrocytes and normal blood monocytes. The 3 methods were compared in terms of time requirements and sensitivity. Erythrophagocytosis required more time to perform (2 h) than did rosette tests (30 min) but less than minimum 51Cr release assays (5.5 h). Erythrophagocytosis was 20-fold more sensitive than either of the other 2 procedures. Results obtained with purified IgG anti-D and with antibodies induced by transfusion or pregnancy were similar.