EXTREME SENSITIVITY OF SOME INTESTINAL CRYPT CELLS TO X AND GAMMA-IRRADIATION
EXTREME SENSITIVITY OF SOME INTESTINAL CRYPT CELLS TO X AND GAMMA-IRRADIATION
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DOI：
10.1038/269518a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
POTTEN, CS
中科院分区：
文献类型：
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作者：
POTTEN, CS
辐射的破坏性影响已经研究了80年。大多数技术都涉及观察存活的细胞，这些细胞往往是组织中更具抵抗力的细胞。假设结果代表组织中的所有细胞，则得出许多结论。另一方面，Cheng和Leblond使用氚化胸苷（3 HTdR）杀死在小海马隐窝中合成DNA的细胞1。他们的实验中有两个令人惊讶的特点几乎没有引起评论。首先，极低剂量（每只小鼠40-50 μCi）的3 HTdR可导致可测量的细胞杀伤，其次，杀伤（从标记的类寄生虫2吞噬体1的存在中可见）在整个隐窝中不是随机的，而是选择性地发生在隐窝基部，S中的细胞相对较少（参考文献3和4），干细胞可能位于此处1，3-5。本文报告了在全身X射线或γ射线照射后，在腺窝底部可发现超敏细胞，并描述了这些死亡细胞产生和消失的时间顺序及其剂量-反应关系。
THE destructive effects of radiation have been studied for 80 yr. Most techniques involve looking at the surviving cells, which tend to be the more resistant cells of the tissue. On the assumption that the results are representative of all cells in the tissue, many conclusions have been drawn. On the other hand, Cheng and Leblond have used tritiated thymidine (3HTdR) to kill cells synthesising DNA in the crypts of the small intestine1. Two surprising features of their experiments have provoked little comment. First, very low doses (40–50 µCi per mouse) of3HTdR caused measurable cell killing and second, the killing (evident from the presence of labelled apoptotic-like2phagosomes1) was not random throughout the crypt but occurred selectively at the crypt base where relatively few cells are in S (refs 3 and 4) and where the stem cells are presumably located1,3–5. I report here that the presence of hypersensitive cells at the base of the crypts can be demonstrated after whole-body X or γ irradiation, and to describe the time sequence for the production and loss of these killed cells together with their dose-response relationship.