Distribution of Ultrabithorax proteins in Drosophila
Distribution of Ultrabithorax proteins in Drosophila
复制标题
Ultrabithorax蛋白在果蝇中的分布
作者：
Robert A. H. White;M. Wilcox
我们用单克隆抗体检测了超双胸（Ubx）蛋白在果蝇胚胎和成虫盘中的分布。Ubx蛋白是核蛋白，在神经系统、表皮和中胚层中显示空间受限的分布。标记在中线细胞中从第一胸节（T1）延伸到第八腹节（A8），在腹神经系统和表皮中从T2延伸到A8，在体细胞中从A1延伸到A8。在神经系统和表皮中，标记的模式表现出重复单元，Ubx元节，与节段重复单元异相。至少在表皮中，这种重复单位似乎在前-后室边界之间延伸，并由后室和随后的前室组成。在神经系统和表皮中最显著标记的后节是包括T3的后部区域和A1的前部区域的后节。在每个变分粒内，细胞核被不均匀地标记。在T3成虫盘的细胞核中也可以看到明显的标记异质性。
We have used a monoclonal antibody to examine the distribution of Ultrabithorax (Ubx) proteins in Drosophila embryos and imaginal discs by immunofluorescence. Ubx proteins are nuclear and show a spatially restricted distribution in the nervous system, epidermis and mesoderm. Labelling extends from the first thoracic segment (T1) to the eighth abdominal segment (A8) in the midline cells, from T2 to A8 in the ventral nervous system and epidermis and from A1 to A8 in the somatic mesoderm. In the nervous systems and epidermis the patterns of labelling exhibit a repeat unit, the Ubx metamere, that is out of phase with the segmental repeat unit. At least in the epidermis this repeat unit appears to extend between anterior‐posterior compartment boundaries and consists of a posterior compartment together with the succeeding anterior compartment. The most prominently labelled metamere in the nervous system and epidermis is that comprising the posterior region of T3 and the anterior region of A1. Within each metamere the nuclei are heterogeneously labelled. Clear heterogeneity of labelling is also seen amongst the nuclei of the T3 imaginal discs.